Which of the following would be the corresponding amino acid sequence that would be translated as a protein product of the following segment of DNA? A genetic code table is provided.   5’ – GGATAGGGGTAA – 3’ 3’ – CCTATCCCCATT – 5’ A. Leu – Pro – Leu – Ser B. Gly – Tyr – Gly – Ser C. Leu – Pro – His – Arg D. Gly – Asp – Ala – Val

1. Working with a partner, come up with as many similarities as you can between prokaryotic gene expression and eukaryotic gene expression. 2. Working with a partner, come up with as many differences as you can between prokaryotic gene expression and eukaryotic gene expression.

In both eukaryotes and prokaryotes, all RNAs that are transcribed from DNA and translated into protein (i.e. mRNAs) contain which of the following? a 5’ untranslated (UTR) region (UTR), a 3’ UTR, a start codon for translation, a stop codon for translation, codons a 5’ untranslated (UTR) region (UTR), a 3’ UTR, a start codon for translation, a stop codon for translation, codons, introns, and exons a 5’ untranslated (UTR) region (UTR), a 3’ UTR, a start codon for translation, a stop codon for translation, codons, and anticodons

1.  Transcription begins at a RNA polymerase-binding site called a _____.   A.  ribosome B.  replicator C.  inducer D.  promoter 2.  In Eukaryotes, following transcription, the intron sequences are cut out of the primary transcript and the ends are joined again prior to its use in protein synthesis. This processing of the RNA is called RNA __________.   A.  modification B.  translation C.  splicing D.  transcription 3. Which of the following is not true about eukaryotic DNA?  A. It is an exceedingly long and fragile molecule. B. It is packaged into successively compact formations. C. The entire molecule has encoded information for protein synthesis. D. In the condensed form, it is transcriptionally inactive. E. It must be unpackaged before it can be transcribed into RNA.

4. The "one gene-one enzyme" hypothesis was proposed by A 4. The "one gene-one enzyme" hypothesis was proposed by  A. Watson and Crick. B. Griffith. C. Garrod. D. Franklin. E. Beadle and Tatum.   5. Amino acids are transported to the ribosome for use in building the polypeptide by  A. mRNA molecules. B. tRNA molecules. C. DNA polymerase molecules. D. rRNA molecules. E. DNA ligase molecules.   

6. b) You plate the mutagenized cells on a master plate so that they form specific colonies (each represented by a solid dot in the diagram below). What are the differences in the media among the 3 plates, above? Explain, briefly what the media contains. Given only the data above, which colonies represent Glu auxotrophs? Colonies G, H and I are formed only on the master plate. How can you explain this observation?

7. Given the sentence "THE FAT CAT ATE THE RED RAT," which of the following would represent a frameshift mutation?  A. THE FAT CAT ATE THE RED RAT B. THE CAT ATE THE RED RAT C. THE FAC ATA TET HER EDR AT D. THE FAT RAT ATE THE RED RAT 8. Base “wobble” refers to: A. alternative RNA splicing B. instability of the DNA molecule C. the ability of the same tRNA anticodon to recognize different codons D. the high mutability of certain genes   9. If you wanted to block transcription of a group of functionally related genes in a prokaryote, you could place an obstacle  A. upstream of both the promoter and operon. B. downstream of both the promoter and operon. C. place an obstacle downstream of the promoter, but upstream of the operon. D. place an obstacle upstream of the promoter, but downstream of the operon.