pCMV mCherry Rluc eGFP IRES Fluc2 EF1α CXCR4-eGFP A B C

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pCMV mCherry Rluc eGFP IRES Fluc2 EF1α CXCR4-eGFP A B C Additional file 1. Schematic diagram of vector construct . (A) Retroviral vector for double reporter gene for making MSC/Fluc2. (B) Retroviral vector of CXCR4 containing double reporter gene for making MSC-CXCR4/Fluc2. (C) Lentiviral particles for double reporter gene containing Rluc and mCherry for MDAMB-231/Rluc.

MSC MSC-CXCR4/Fluc2 Additional file 2. eGFP analysis by FACS of MSC/CXCR4-Fluc2 cells. The gating strategy included two successive gates: a first gate on SSC-H and FSC-H to select cells (P1), and for the histogram another gate (P2) drawn for confirm the GFP positive cells. The experiments were performed in triplicate used for analyzing the means ± standard deviation (SD).