Background and objectives

Slides:



Advertisements
Similar presentations
5/20/2015ISAP Is the cellular accumulation of antibiotics a predictive factor for intracellular efficacy ? Sandrine Lemaire Université catholique.
Advertisements

PK/PD of Antibiotics in relation to resistance Otto Cars MD Department of Medical Sciences Infectious diseases Uppsala University Sweden.
Hunting intracellular bacteria with antibiotics
Bacterial persistence
Setting-up a model of intracellular infection by Pseudomonas aeruginosa for the pharmacodynamic evaluation of antibiotic activity. J. Buyck1, O. Jolois2,
studies with Caco-2 cells and THP-1 macrophages
Activities of Daptomycin (DAP), Vancomycin (VAN) and Linezolid (LDZ) alone or in combination with Fusidic acid (FUS) in an in vitro dynamic model of.
A-052 Antibiotic (AB) activity against Pseudomonas aeruginosa (PA) with Normal or Mucoïd Phenotypes in an Artificial Sputum Medium (ASM) in vitro Biofilm.
Activity of tobramycin in combination with clarithromycin
Introduction Results Aim of the study Methods References Conclusion
Antifungal activity (MIC/MFC) against Candida spp.
Comparison of the intracellular and extracellular activities of approved and novel antistaphylococcal antibiotics using a pharmacodynamic model exploring.
Intracellular antibiotics and Listeria monocytogenes
Françoise Van Bambeke & Paul M. Tulkens
A small molecule candidate for antibiotic co-therapy in the fight against persistence Valerie Defraine1, V. Liebens1, T. Swings1, R. Corbau2, A. Marchant2,
In vitro pharmacodynamic models for the study of antibiotic activity against bacterial biofilms Françoise Van Bambeke, PharmD, PhD Pharmacologie cellulaire.
Mix with THP-1 macrophages Overnight bacterial suspension
Frédéric Peyrusson, Françoise Van Bambeke, Paul M. Tulkens
Type III secretion system (TTSS) INTRODUCTION AND OBJECTIVES
In vitro susceptibility of S
Activity of 9 antibiotics against intracellular forms of S. pneumoniae
P1058 Intracellular activity of antibiotics against a stable small colony variant (SCV) isolated from a CF patient in a model Calu-3 human airway epithelial.
Expression of Efflux system
Revealing moxifloxacin activity against biofilms of S
Can efflux confer high levels resistance to meropenem (MEM) in Pseudomonas aeruginosa (Pa) clinical isolates? H. Chalhoub1, H. Rodriguez-Villalobos2,
Introduction & Purpose Results Conclusions
FUS, VAN and LZD injected twice (T0 and T12); DAP injected once (T0).
This poster will be made available for download after the meeting at :
Influence of antibiotic treatments on gene expression of RND efflux pumps in successive isolates of Pseudomonas aeruginosa collected from patients with.
Introduction Results Aim Methods References Conclusion
Co-medications Improve Moxifloxacin (MXF) Activity in Models of Pneumococcal Naïve and Induced Biofilms (BF) N. M. Vandevelde1, M. Van Obbergh1, P.M. Tulkens1,
Comparative in vitro activity of temocillin and other β-lactams
Activity and Pharmacodynamic (PD) Evaluation of Ceftazidime-Avibactam (CAZ-AVI) against Extracellular and Intracellular Forms of CAZ-susceptible and CAZ-resistant.
Inhibitors of type three secretion system [TTSS] protect against Pseudomonas aeruginosa cellular toxicity by inhibiting the transcription of TTSS Mailing.
Table 2: Percentage of cross resistance among tested antibiotics
Emilien Drouot, Paul M. Tulkens, Françoise Van Bambeke
Julien Buyck, Paul M. Tulkens and Françoise Van Bambeke
Julien Buyck, Paul M. Tulkens and Françoise Van Bambeke
Extracellular activity Intracellular activity
Antibiotics in bone and joint infections
Abstract Results Methods Background Conclusions References
cellular accumulation (24h) Cstatic (LZD/RDZ ratio)
INTRODUCTION AND OBJECTIVES MATERIAL AND METHODS RESULTS CONCLUSION
Apoptosis induced by aminoglycosides (AGs) in cultured cells : comparison between gentamicin (GEN) and amikacin (AMK) using incubated and electroporated.
Incubation (with antibiotic)
52 - Antimicrobials Radezolid (RX-1741), a Novel Oxazolidinone, Accumulates Extensively within Human Macrophages and PMNs and Shows Activity towards.
Of a Pig-related ST-398 Methicillin-Resistant S. aureus (A-MRSA)
EV0626 Cellular pharmacokinetics of gepotidacin (GSK )
A-045 Identification of the Efflux Transporter of Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells Béatrice Marquez, Nancy.
Abstract Results Aim of the study Methods Background Conclusions
Difference log CFU from time 0
DISCUSSION AND CONCLUSION
INTRODUCTION METHODS CONCLUSIONS REFERENCES
Introduction Results Materials and Methods Conclusions
1200 Brussels - Belgium Temocillin is not substrate for OprD2 porin from Pseudomonas aeruginosa H. Chalhoub1,
Electron microscopy: intracellular infection of THP-1 by S. aureus
Log extracellular concentration (mg/L)
Susceptibility of Pseudomonas aeruginosa (P. a
Activity of Moxifloxacin against Staphylococcus aureus in Models of Persistent Infections (Intracellular Survival, Biofilms) Mailing address: P.M. Tulkens.
M.P. Mingeot-Leclercq, P.M. Tulkens, F. Van Bambeke
P.L. Toutain VMAS symposium/workshop Uppsala 13 December 2017
Activities of antistaphylococcal antibiotics towards the extracellular and intraphagocytic forms of Staphylococcus aureus isolates from a patient with.
Intracellular antibiotics
Intracellular models of infection to evaluate antibiotic activity
Activities of antistaphylococcal antibiotics towards the extracellular and intraphagocytic forms of Staphylococcus aureus isolates from a patient with.
Setting-up of a 24 h model to evaluate the activity of antibiotics against intracellular forms of S. aureus infection C. Seral, M. Barcia-Macay, F. Van.
“Pulmonary infections”
Are intracellular drug concentrations relevant for efficacy
Relative potency of Drugs
Presentation transcript:

Background and objectives Pharmacological effects of tobramycin/macrolides combination on extracellular and intracellular infection by Pseudomonas aeruginosa Julien M. Buyck1, Hamidou Traore2, Francis Vanderbist2, Paul M. Tulkens1 and Françoise Van Bambeke1 1Pharmacologie cellulaire et moléculaire & Louvain Drug Research Institute, Brussels, Belgium. 2 Laboratoires SMB, Brussels, Belgium Mailing address: F. Van Bambeke av. Mounier 73, B1.73.05 1200 Brussels – Belgium, francoise.vanbambeke@uclouvain.be Background and objectives Results P. aeruginosa (PA) is capable of invading epithelial and phagocytic cells (1), which may play an important role in the initiation and persistence of infections. PA is reported as intrinsically resistant to several antibiotics, including macrolides (ML). Yet ML are currently used with success in clinics to treat cystic fibrosis patients with chronic PA infection. We have shown that ML regain activity against PA in media relevant of the in vivo environment (serum, bronchoalveolar lavage fluid [BAL]) or in culture medium for eukaryotic cells (RPMI-1640) (2). This study examines the activity of combinations of tobramycin (TOB) and macrolides (azithromycin [AZM] or clarithromycin [CLR]) against intracellular PA (24h infection in THP-1 phagocytic cells) in comparison with extracellular PA grown in broth (MHB) or in RPMI-1640 (RPMI). Activity of antibiotics against extracellular (blue) and intracellular (orange) P. aeruginosa. 1. MICs of antibiotics 2. Dose effect curves Antibiotics MIC (mg/L) MHB RPMI-1640 Tobramycin 1 4 Azithromycin 256 16 Clarithromycin >512 64 MICs of ML were much lower in RPMI than in MHB MIC of TOB was 2 dilutions higher in RPMI Extracellularly ML were poorly potent (EC50 very high) but were 6- fold lower in RPMI than in MHB TOB relative potency was higher in MHB than in RPMI Intracellularly Emax and potency were reduced for TOB Materials and Methods 3. Pharmacological parameters Bacterial strain and susceptibility testing. P. aeruginosa strain ATCC PAO1 was used. MICs were measured by microdilution in MH broth or in eucaryotic cell media RPMI-1640 supplemented with 10% of foetal calf serum. Pharmacodynamics of antibiotics alone. Extracellular activity was measured in MHB or in RPMI-1640 (+ 10 % foetal calf serum); intracellular activity was measured in a model of PAO1-infected THP-1 cells. PD parameters (Emax [max CFU decrease extrapolated for infinitely large concentration]; EC50 [concentration for which E = ½ Emax]) were calculated from the Hill equation of the dose-response. Pharmacodynamics of combinations. We used the Fractional Maximal Effect (FME) method, where antibiotic concentrations to be tested are calculated from EC50 and Emax to obtain 0.1, 0.3, 0.5, 0.7, 0.9-fold the Emax. Activity was measured for combinations at concentration of antibiotic A and antibiotic B giving rise to of 0.1:0.9, 0.3:0.7, 0.5:0.5, 0.7:0.3; 0.9:0.1 effect ratio (3). Fractional Maximal Effect (observed /theoretical effect): synergy > 1; additivity ~ 1; indifference: < 1; antagonism: < effect of best antibiotic alone Antibiotics Extracellular (MHB) (RPMI) Intracellular Emaxa (log cfu) EC50b (mg/L) EC50b (mg/L) Tobramycin > -4.5 0.4 8.9 -1.1 3.6 Azithromycin 38.9 -3.0 6.1 -3.3 72.3 Clarithromycin -2.7 510.5 -3.9 82.0 -4.0 851.7 a relative maximal efficacy: CFU decrease (log10 units) at time 24 h from the corresponding original inoculum, as extrapolated for an infinitely large antibiotic concentration b drug concentration giving a response half-way between E0 and Emax Opsonization (1 h, 37°c) 9 mL RPMI + 1 mL human serum Phagocytosis (2 h) Activity of combinations against extracellular (upper panel) and intracellular (lower panel) P. aeruginosa. 750,000 cells/mL MOI 10 Extracellular Wash GEN 100 µg/ml (1 h) 2. Dose effect curves 1. MICs of combinations AZM / TOB 0.9/0.1 0.7/0.3 0.5/0.5 0.3/0.7 0.1/0.9 895.05 232.05 99.45 42.62 11.05 mg/L 0.34 1.30 3.03 7.06 27.23 0.0 0.5 1.0 1.5 2.0 2.5 3.0 AZM TOB Fractional maximal effect (FME) CLR / TOB 329.13 85.33 36.57 15.67 4.06 CLR 72.20 18.75 8.06 3.4 2 0.9 5 1. 4 3 5.30 12.43 29.96 111.43 890 .13 230 . 8 98.9 7 42.4 10. 06 1 .30 12.4 28.9 6 111.4 RPMI MHB Intracellular 5 to 7x105 CFU/mg prot. Time 0 Combinations MIC (mg/L) MHB RPMI TOB/AZMa 0.5 TOB/CLRa a for a combination TOB/macrolide of 10:2 (w/w) Incubation (with antibiotic) (24 h) 3. Pharmacological parameters Combinations FME MHB RPMI Intracellular TOB/AZMa 1.31 1.44 1.09 TOB/CLRa 1.24 1.47 1.12 a for a combination TOB/macrolide of 0.9: 0.1 CA= FMEA.EC50A 1-FMEA CB= FMEB.EC50B 1-FMEB Extracellularly Combinations showed lower MICs than those of AB alone All combinations were slightly synergistic (FME>1) Intracellularly Combinations globally rather show additive effets Conclusions References The relative potency of antibiotics towards PA is reduced intracellularly as compared to that observed in broth. For ML, this suggests that the intracellular medium, in contrast to other eukaryotic environments (like BAL, serum, or RPMI-1640), does not allow them to recover their activity against PA. Yet, combining them with TOB, a major antipseudomonal agent, proves synergistic or additive against extracellular and intracellular bacteria. This warrants further investigations to extend this observation to clinical isolates, including particular phenotypes frequent in cystic fibrosis patients (small colony variants, mucoïdal). (1) Kierbel et al., Mol. Biol. Cell 2005,16: 2577-85 (2) Buyck et al., Clin. Infect. Dis. 2012, 55:534-542 (3) Nguyen et al., AAC 2009, 53: 1443–9 ACKNOWLEDGEMENTS: We thank M.C. Cambier, V. Mohymont and C. Misson for excellent technical assistance. This work was supported by the BioWin program (Region Wallone; Belgium). This poster will be made available for download after the meeting: http://www.facm.ucl.ac.be/posters.htm