SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway Zhang Meng 1 ;Deng Yong-Ning 1 ;Zhang Jing-Yi 1 ;Liu Jie 1 ;Li Yan-Bo 1 ;Su Hua 2 ;Qu Qiu-Min 1 ; 1 Department of Neurology, the First Affiliated Hospital of Xi#cod#x02019;an Jiaotong University, Xi#cod#x02019;an, China. ; 2 Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, California, USA. ; Figure 3. SIRT3 prevents rotenone-induced cell death in human neuroblastoma SH-SY5Y cells by upregulating autophagy. The SH-SY5Y cells with or without SIRT3 overexpression cultured in non-serum growth medium were treated with 60 #cod#x003BC;M rotenone for 24 h. Five millimolar 3-MA was added 1 h before rotenone. A Then, the treated cells were subjected to the MTT assay. Bar graph shows the quantification of cell viability determined by MTT assay in WT, WT+Rot, WT+3-MA, Vehicle, Vehicle+Rot, Vehicle+3-MA, SIRT3+, SIRT3+Rot, and SIRT3+Rot+3-MA groups. Mean #cod#x000B1; SEM, n=3. #: P 0.001 vs. Vehicle+Rotenone. After treatment as described above, cells were harvested, stained with 7-AAD Y-axis and PE-Annexin V X-axis. Scatter diagram B and bar graph C show the flow cytometric analysis of staining from WT, WT+Rot, WT+3-MA, Vehicle, Vehicle+Rot, Vehicle+3-MA, SIRT3+, SIRT3+Rot, and SIRT3+Rot+3-MA groups. The apoptotic rate= #cod#x0005B;AnnexinV-PE+7-AAD-cells + AnnexinV-PE+7-AAD+cells#cod#x0005D;total cells#cod#x000D7;100#cod#x00025;. Mean #cod#x000B1; SEM, n=3. Rot=Rotenone. #cod#x003B4;: P 0.001 vs. Vehicle+Rotenone. null,null,0(0),null-null. Doi:10.14336/AD.2017.0517