Magdaléna Netuková, Yveta Urbanová, Josef Šach, Pavel Kuchynka

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Presentation transcript:

The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague Magdaléna Netuková, Yveta Urbanová, Josef Šach, Pavel Kuchynka Ocular Tissue Bank OTB01/The International Eye Bank of Prague, Czech Republic Dpt. of Ophthalmology Vinohrady Teaching Hospital Prague, Czech Republic All authors have No finacial interest

Aim of the study To evaluate quality of Pre-Cut corneas for DSAEK prepared in the Interantional Eye Bank of Prague To analyse possible complications during preparation of the Pre-Cut corneas. To analyse possible complications after preparation of Pre-Cut corneas

Methods - preparation Pre-Cut corneas were prepared from 30 corneas – not suitable for surgery All Pre-Cut corneas were preapred in „Clean rooms“ by certified staff members All Pre-Cut corneas were prepared using standardized method for DSAEK – with Moria microkeratome Moria microkeratome Ljfpqejqoj tpoj Jkpavjpjp ojp

No major technical problems Epithelial abrasion Mounting Pachymetry No major technical problems during preparation Cut Free flap evaluatin Free flap reposition Dismounting

Methods - Evaluation All Pre-Cut corneas were evaluated using Slit lamp Specular microscopy Selected Pre-Cut corneas were screend for Microbial comtamination Ljfpqejqoj tpoj Jkpavjpjp ojp

Results - Slit lamp evaluation All 30 Pre-Cut corneas were evaluated using slit lamp In 28 Pre-Cut corneas we found good position of the corneal free flap In 2 cases we foud dislocation of the free flap into the medium No obvious stromal or endotehelial defects or opacity were detected Incorrect position of the free flap Ljfpqejqoj tpoj Jkpavjpjp ojp Correct position of the free flap

Results Specular microscopy evaluation The average endothelial cell censity prior PreCut was 2709±483(SD) cells/mm2, The average endothelial cell censity after PreCut was 2623±407(SD) cells/mm2 We found no significant endothelial cell loss after preparation. Corneal endothelium after Pre-Cut

Endothelial Cell Density Ljfpqejqoj tpoj Jkpavjpjp ojp

Resuts - Microbiology 5 randomly selected samples of medium and corneal swaps were microbiologically tested for microbial and fungal contamination – all results were negative no medium showed signs of bacterial contamination 1 week after preparation of the Pre-Cuts.

Summary Preparation Evaluation In our serie we experienced no major technical problems The procedure is straight forward with relatively short learning curve Evaluation In our serie evaluation of prepared tissue showed high quality and high safety

Conclusion We believe that our eye bank prepares Pre-Cut corneas for lamelar keratoplasty of very high quality and safety We also believe that in the near future Pre-Cut corneas will represent majority of tissues provided by our eye bank

Thank you International Eye Bank of Prague Srobarova 50 Czech Republic e-mail:eyebank@fnkv.cz Tel: +420 26716 3678, +420 26716 2267