Development of a simple one-pot extraction method for various drugs and metabolites of forensic interest in blood by modifying the QuEChERS method Shuntaro.

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Presentation transcript:

Development of a simple one-pot extraction method for various drugs and metabolites of forensic interest in blood by modifying the QuEChERS method Shuntaro Matsuta, Keiko Nakanishi, Akihiro Miki, Kei Zaitsu, Noriaki Shima, Tooru Kamata, Hiroshi Nishioka, Munehiro Katagi, Michiaki Tatsuno, Kento Tsuboi, Hitoshi Tsuchihashi, Koichi Suzuki Forensic Science International Volume 232, Issue 1, Pages 40-45 (October 2013) DOI: 10.1016/j.forsciint.2013.06.015 Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 GC–MS chromatograms obtained from a whole blood sample spiked with 8 drugs at 0.5μg/mL each, processed by the optimized method. AP, amphetamine; MA, methamphetamine; PB, Phenobarbital; FN, Flunitrazepam; ZP, zolpidem; BZ, brotizolam; PM, promethazine; and CP, chlorpromazine. Forensic Science International 2013 232, 40-45DOI: (10.1016/j.forsciint.2013.06.015) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 LC–MS chromatograms obtained from a whole blood sample spiked with 13 drugs and metabolites at 0.5μg/mL each, processed by the optimized method. AP, amphetamine; MA, methamphetamine; ZC, the carboxylate-form major metabolite of zolpidem; AF, 7-aminoflunitrazepam; PB, phenobarbital; HT, α-hydroxytriazolam; HB, α-hydroxybrotizolam; FN, flunitrazepam; ZP, zolpidem; TZ, triazolam; BZ, brotizolam; PM, promethazine; and CP, chlorpromazine. Forensic Science International 2013 232, 40-45DOI: (10.1016/j.forsciint.2013.06.015) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Recoveries of the 13 drugs and metabolites by the modified QuEChERS method and liquid–liquid extraction (LLE). Dark-gray, the modified QuEChERS method; white, LLE with chloroform–isopropanol (3:1, v/v), at pH 4.5; and light-gray, LLE with chloroform–isopropanol (3:1, v/v) at pH 9. AP, amphetamine; MA, methamphetamine; ZC, the carboxylate-form major metabolite of zolpidem; AF, 7-aminoflunitrazepam; PB, phenobarbital; HT, α-hydroxytriazolam; HB, α-hydroxy-brotizolam; FN, flunitrazepam; ZP, zolpidem; TZ, triazolam; BZ, brotizolam; PM, promethazine; CP, chlorpromazine. Forensic Science International 2013 232, 40-45DOI: (10.1016/j.forsciint.2013.06.015) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 GC–MS chromatograms obtained from a forensic blood specimen, processed by the modified QuEChERS method. Forensic Science International 2013 232, 40-45DOI: (10.1016/j.forsciint.2013.06.015) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 LC–MS chromatograms obtained from a forensic blood specimen, processed by the modified QuEChERS method. AP, amphetamine; MA, methamphetamine. Forensic Science International 2013 232, 40-45DOI: (10.1016/j.forsciint.2013.06.015) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions