A modified Raman multidimensional spectroscopic signature of blood to account for the effect of laser power  Gregory McLaughlin, Igor K. Lednev  Forensic Science International  Volume 240, Pages 88-94 (July 2014) DOI: 10.1016/j.forsciint.2014.04.021 Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Top – comparison of a Raman spectrum of dried blood obtained under high power (red) and low power (blue). Bottom – the normalize peak intensity of 1250cm−1 of spectra under various laser power settings (20× objective) and under continuous exposure for three hours (50× objective) with 10% laser power. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Left panel – five low power blood spectra (blue) overlapped with the reconstructed spectrum (green) using the original blood signature. Right panel – five low power blood spectra (blue) overlapped with the reconstructed spectrum (green) using the sweat signature. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Raman spectra of the new low power spectral component (a), original blood component 1 (b) and original blood component 2 (c). Dashed line is at 1229cm−1. Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Left panel – five low power blood spectra (blue) overlapped with the reconstructed spectrum (green) using the combined blood signature. Right panel – five low power blood spectra from external validation (blue) overlapped with the reconstructed spectrum (green) using the combined blood signature. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Four experimental Raman spectra (blue) of semen (1), sweat (2), vaginal fluid (3) and saliva (4), overlapped with the reconstructed spectrum (green) using the combined blood signature. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 6 Comparison of the Raman spectra of blood plasma, serum albumin and fibrin to the loading of component #2 (original signature). Forensic Science International 2014 240, 88-94DOI: (10.1016/j.forsciint.2014.04.021) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions