Cell Physiol Biochem 2016;39: DOI: /

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Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes Cell Physiol Biochem 2016;39:81-88 - DOI:10.1159/000445607 Fig. 1. Raman spectra of erythrocyte suspensions measured after 20-min exposure to air (1) or oxygen-free gas mixture (2). © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes Cell Physiol Biochem 2016;39:81-88 - DOI:10.1159/000445607 Fig. 2. Relationship between increments of hemolysis and ATP release in human erythrocytes. Accumulation of extracellular hemoglobin (A) and ATP (B) triggered by 20-min incubation of human erythrocytes in normoxic and hypoxic conditions. Means ± S.E. obtained in 17 experiments with blood from different donors are shown. The total content of hemoglobin and ATP was taken as 100%. *, ** p < 0.02 and 0.001 compared to normoxia, respectively. Mean values of increments triggered by hypoxia are shown by hutched columns. C. Linear regression analysis of increments of hemolysis and ATP release triggered by hypoxia. rxy is the Pearson product-moment correlation coefficient and p is the measure of correlation's significance estimated by the Student's t-test. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes Cell Physiol Biochem 2016;39:81-88 - DOI:10.1159/000445607 Fig. 3. Effect of hypoxia on hemolysis and the content of membrane bound proteins in human erythrocytes. A - Hemolysis of erythrocytes under conditions of normoxia and hypoxia. Means ± S.E. from 4 independent experiments are shown. The total content of hemoglobin was taken as 100%. * p<0.01. B - Representative PAGE of erythocyte ghost obtained in normoxic and hypoxic conditions. C - the relative protein X content in erythrocyte membranes obtained in control (open bars) and hypoxic (black bars) conditions. Means ± S.E. from 4 independent experiments are shown. The content of band 3 protein was taken as 100%. * p<0.01. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0