Application of Ultraviolet/Visible Molecular Absorption Spectrometry

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Presentation transcript:

Application of Ultraviolet/Visible Molecular Absorption Spectrometry Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Application of Ultraviolet/Visible Molecular Absorption Spectrometry Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14

Quantitative Analysis by Absorption Measurement Scope Application to absorbing species Application to non-absorbing species Procedural Details Selection of wavelength Variables that influence absorbance Cleaning and handling of cells Determination of the relationship between absorbance and concentration The standard addition method Cs = A1CxVx/((A2-A1)*Vx)

Standard Addition Method 1.000 0.800 m =0.03820 Absorbance 0.600 0.400 b = 0.2412 0.200 Vs = -6.35 -10.0 0.0 10.0 20.0 Volume standard solution mL

Typical Photometric Titration Curves Volume of titrant Absorbance es = ep = 0 et >0 Absorbance Volume of titrant es > 0 ep = et = 0 Absorbance ep > 0 es = et = 0 Volume of titrant Volume of titrant Absorbance es > et > 0 ep= 0 Volume of titrant Absorbance et > ep > 0 es = 0 Absorbance Volume of titrant es = 0 ep > et > 0

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