Copyright © 2014 American Medical Association. All rights reserved. From: Chronic Lymphocytic LeukemiaA Clinical Review JAMA. 2014;312(21):2265-2276. doi:10.1001/jama.2014.14553 Figure Legend: Characteristic Histologic Features and Immunophenotype of Chronic Lymphocytic LeukemiaA, Peripheral blood smear from a patient with chronic lymphocytic leukemia (CLL) that shows scattered smudge cells and small lymphoid cells with clumped chromatin typical of CLL (Wright-Giemsa, original magnification ×200). B, Histology of lymph node biopsy tissue from the same patient showing diffuse proliferation of small lymphoid cells with occasional pale areas corresponding to proliferation centers (hematoxylin-eosin, original magnification ×100). Inset shows a paraimmunoblast (large cell with prominent nucleoli) within 1 proliferation center (arrowhead) (hematoxylin-eosin, original magnification ×400). Flow cytometry plot (from a different patient with CLL) depicting CLL population in red expressing (C) CD19 (B-cell marker) and CD5, in addition to (D) CD23 with (E) lambda light chain restriction; nearly all CLL cells shown in red express uniform lambda light chain as depicted on the x-axis. All antigens are tagged with the depicted fluorochromes. The axis scales represent fluorescence intensity. FITC indicates fluorescein isothiocyanate; PE, phycoerythrin; ECD, electron coupled dye; PC5, phycoerythrin-cyanine 5. Figure courtesy of Girish Venkataraman, MD, Department of Pathology, University of Chicago. Date of download: 10/24/2017 Copyright © 2014 American Medical Association. All rights reserved.