Small Animal Ventilator for Hyperpolarized Helium MRI Ashley Anderson III, Micah Brown, Matt Smith, Chris Wegener Client: Dr. Sean Fain Advisor: Dr. Willis Tompkins

Client Requirements MRI compatible ventilator system Deliver 20% O2 and 80% He-3 in same breath Allows constant scanning of the He-3 in MRI rather than every 4 breaths

Background - Helium MRI Newer technique Same MRI scanner Convention is H Here, tuned to Helium (48.6 MHz) Breath of Helium Displays respiratory channels

Project Concept Each syringe feeds off of independent gas reservoirs Gas mixing occurs right before entering small animal

First Generation Prototype Currently in use in animal studies Only using one syringe Not compatible with small syringes for mouse studies

Modifications to G1 Reinforced syringe clips Larger motor Eliminated O2 chaser breath

Calibration Results using manometer testing. Graph shows small deviation from entered volumes.

Second Generation Concept

Second Generation Prototype Created in Solidworks Smaller components Interchangeable syringes Adjustable axle support

Second Generation Prototype Large motor Motor shielding Precision machining Can be disassembled

From Solidworks to Reality

Does it move?

Did we meet client requirements? Gear ratios allow the appropriate 80/20 gas output ratio MRI Safe materials Motor shielded for RF Rat/Mouse compatible

Future Work Calibration testing Software development Volume delivery Timing Software development Simultaneous 3He/O2 delivery Inspired time based on %Insp and RR Valve switching Mixing testing (imaging)

Questions?