Fig. 4. Determination of the optimal dose of compound 48/80 in studying biomarkers for cutaneous neurogenic inflammation (CNI). (A) Reverse transcriptase-

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Fig. 4. Determination of the optimal dose of compound 48/80 in studying biomarkers for cutaneous neurogenic inflammation (CNI). (A) Reverse transcriptase- polymerase chain reaction (RT-PCR) experiments for thymic stromal lymphopoietin (TSLP), nerve growth factor (NGF), and calcitonin gene-related peptide (CGRP), biomarkers for CNI, were performed as described in the MATERIALS AND METHODS. HaCaT cells were treated with different doses of compound 48/80 (1~10 µg/ml) to find out the optimal dose of compound 48/80 in following experiments. (B) Densitometric analysis on the relative density of tested biomarkers are depicted. This picture represents three repetitive experiments of RT-PCR. Fig. 4. Determination of the optimal dose of compound 48/80 in studying biomarkers for cutaneous neurogenic inflammation (CNI). (A) Reverse transcriptase- polymerase chain reaction (RT-PCR) experiments for thymic stromal lymphopoietin (TSLP), nerve growth factor (NGF), and calcitonin gene-related peptide (CGRP), biomarkers for CNI, were performed as described in the . . . Ann Dermatol. 2016 Jun;28(3):344-351. http://dx.doi.org/10.5021/ad.2016.28.3.344