From: Expression Profile of Nerve Growth Factor after Muscle Incision in the Rat Anesthes. 2009;110(1):140-149. doi:10.1097/ALN.0b013e318190bc84 Figure Legend: Fig. 3. 3′3-diaminobenzidine immunohistochemistry for nerve growth factor (NGF) in plantar flexor digitorum brevis muscle. ( A ) NGF immunohistochemistry in sham-operated flexor muscle. ( B ) NGF in nerve-like structures from incised muscle on POD (postoperative day) 1. ( C ) A higher magnification of the rectangular area C in panel B , showing immunoreactivity for NGF within a transverse sectioned nerve-like structure. ( D ) A higher magnification of the rectangular area D in panel B , showing immunoreactivity for NGF within a longitudinal sectioned nerve-like structure. ( E ) NGF immunohistochemistry in skin, fascia, and muscle after preabsorption with blocking peptide. The arrow indicates the incision. Date of download: 10/25/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.