Volume 111, Issue 6, Pages 1691-1696 (June 1997) Detection of Chromosomal Aneuploidy by Interphase Fluorescence In Situ Hybridization in Bronchoscopically Gained Cells From Lung Cancer Patients  Thomas Schenk, MD, Jutta Ackermann, Christoph Brunner, MD, Peter Schenk, MD, Niklas Zojer, MD, Sebastian Roka, MD, Johannes Drach, MD  CHEST  Volume 111, Issue 6, Pages 1691-1696 (June 1997) DOI: 10.1378/chest.111.6.1691 Copyright © 1997 The American College of Chest Physicians Terms and Conditions

FIGURE 1 A: Patient 658: adenocarcinoma of the lung, pleural effusion. Highly hyperdiploid cell showing seven hybridization signals for chromosome 3 (green fluorescence) and eight copies for chromosome 17 (red fluorescence). Note intratumor heterogeneity in comparison with surrounding cells. B: Patient 744: normal cells from bronchial epithelium with disomic results for chromosomes 17 (red fluorescence) and 18 (green fluorescence). Imprint of bronchoscopically gained biopsy specimen shows conserved architecture. C: Patient 744: another group of disomic cells (chromosomes 18, green fluorescence, and 17, red fluorescence). D: Patient 744: nest of four tumor cells (adenocarcinoma). Green hybridization signals represent copies of chromosomes 11, red signals represent copies of chromosome 7. Again, a marked heterogeneity of aneuploid cells is observed. Note that the yellow signal results from overlapping green and red signals. E: Patient 701: brushing: inconspicuous cell from a patient with squamous cell carcinoma: interphase FISH reveals two signals for chromosome 17 (red fluorescence) and four signals for chromosome 18 (green fluorescence). F: green signals representing chromosome 18 in the same nucleus are shown in more detail using a different filter set. C: Patient 172: double color FISH in a highly aberrant tumor cell (squamous cell carcinoma) showing more than 10 signals for chromosome 3 (green signals) and 17 (red signals). Note that not all signals are in the same plane of the focus due to the three-dimensional structure of the nucleus. CHEST 1997 111, 1691-1696DOI: (10.1378/chest.111.6.1691) Copyright © 1997 The American College of Chest Physicians Terms and Conditions