Fig. 2. Post-treatment with isoflurane reduced infarct volume Fig. 2. Post-treatment with isoflurane reduced infarct volume. Brain damage was estimated with TTC staining following one hour of ischemia and 24 h of reperfusion. Animals in the TPA + ISO group received 1.5% isoflurane from the onset of reperfusion for one hour. (A) Representative TTC stained images of brain sections from an MCAO rat. (B) Quantification of infarct volume (n = 5 in each group). MCAO: middle cerebral artery occlusion, TPA: control group, TPA + ISO: isoflurane post-treatment group, TTC: 2,3,5-triphenyltetrazolium chloride. Values shown are means ± SEM. *P value = 0.005 vs. TPA. Fig. 2. Post-treatment with isoflurane reduced infarct volume. Brain damage was estimated with TTC staining following one hour of ischemia and 24 h of reperfusion. Animals in the TPA + ISO group received 1.5% isoflurane from the onset of reperfusion for one hour. (A) Representative TTC stained images of brain sections from an MCAO rat. (B) Quantification of infarct volume . . . Korean J Anesthesiol. 2015 Jun;68(3):281-286. http://dx.doi.org/10.4097/kjae.2015.68.3.281