Supplemental Figure 1 Supplemental Figure 1: Electron Force Microscopy image of a DBS filter incubated with human blood.

Supplemental Figure 2 Supplemental Figure 2: Correlation of technical replicates in version 19 and 21 of the miRBase.

Supplemental Figure 3A n=30 samples p=60 miRNAs Supplemental Figure 3A: Clustering of the lung cancer samples and process controls measured using version 19 of the miRBase.

Supplemental Figure 3B n=18 samples p=40 miRNAs Supplemental Figure 3B: Clustering of the lung cancer samples and process controls measured using version 21 of the miRBase.

Supplemental Figure 3C Supplemental Figure 3C: Correlation matrix of all samples. The process controls with highest correlation to each other can be observed in the lower right corner.

(all miRNAs expressed in at least 75% of all samples) Supplemental Figure 4A p=562 miRNAs (all miRNAs expressed in at least 75% of all samples) Supplemental Figure 4A: Averaged normalized expression intensity versus variance of expression intensity of process controls colored by the GC content.

(all miRNAs expressed in at least 75% of all samples) Supplemental Figure 4B p=562 miRNAs (all miRNAs expressed in at least 75% of all samples) Supplemental Figure 4B: Averaged normalized expression intensity versus variance of expression intensity of process controls colored by the miRBase version where each miRNA has been introduced to miRBase.