Reproduced with permission from Cronin M et al

Slides:



Advertisements
Similar presentations
Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of.
Advertisements

Ligation In-situ Hybrdization Christopher Itoh 1, Joel Credle 1, Rajni Sharma 2, H. Benjamin Larman 1 1 Department of Immunopathology, Johns Hopkins University.
Figure 1 Food, nutrition, obesity, physical activity, and the cellular processes linked to cancer Figure 1 | Food, nutrition, obesity, physical activity,
Figure 1 The heterogenous landscape of triple-negative breast cancer
Nat Clin Pract Endocrinol Metab 3: 279–289 doi: /ncpendmet0447
Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,
Figure 1 Comparison of tumor size at time of excision between axillary node-negative, axillary node-positive, and distant metastasis subgroups of basal-like.
Figure 3 Response after appearance of a new lesion
Nat. Rev. Clin. Oncol. doi: /nrclinonc
Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,
Figure 5 Mutational heterogeneity in oesophageal and gastric cancer
Molecular classification of mature aggressive B-cell lymphoma using digital multiplexed gene expression on formalin-fixed paraffin-embedded biopsy specimens.
سرطان الثدي Breast Cancer
Application of Selected Reaction Monitoring for Multiplex Quantification of Clinically Validated Biomarkers in Formalin-Fixed, Paraffin-Embedded Tumor.
Figure 5 Schematic illustration of different clinical trial designs
Nat. Rev. Clin. Oncol. doi: /nrclinonc
A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin  Dimitri Talantov, Jonathan Baden,
Masanobu Ishii et al. BTS 2017;2:
Nat. Rev. Clin. Oncol. doi: /nrclinonc
Figure 1 Underreporting of treatment-related toxicities by physicians, relative to patients with either advanced-stage lung cancer, or early-stage breast.
Characterization of microRNA transcriptome in tumor, adjacent, and normal tissues of lung squamous cell carcinoma  Jun Wang, MD, PhD, Zhi Li, MD, PhD,
Figure 2 Therapeutic targeting of the PI3K/AKT/mTOR pathway
Figure 4 Effects of delaying cardioprotective medications after anthracycline administration Figure 4 | Effects of delaying cardioprotective medications.
Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5′ Nuclease Quantitative Reverse Transcription-Polymerase Chain.
PD-1 and LAG-3 expression in MSI and MSS colorectal cancer specimens.
Nat. Rev. Neurol. doi: /nrneurol
Figure 1 NIR fluorescence is more suitable for
Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating lymphocytes. Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating.
Martin Steinau, Sonya S. Patel, Elizabeth R. Unger 
High-throughput analysis of informative CpG sites for the four studied tumor suppressor genes (A-D). High-throughput analysis of informative CpG sites.
Figure 3 Clinical trial design in charged-particle therapy (CPT)
Nat. Rev. Clin. Oncol. doi: /nrclinonc
Figure 2 Key features of gastric cancer subtypes according to The Cancer Genome Atlas (TCGA) Figure 2 | Key features of gastric cancer subtypes according.
Intravenous delivery of reovirus to primary and secondary brain tumors
Figure 2 Frequency and overlap of alterations
Figure 2 Variations between planned and delivered doses of radiation
Reliable Transcript Quantification by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Primary Neuroblastoma Using Normalization to Averaged.
Coexistence of Tyrosine Kinase Inhibitor-Sensitizing and Resistant EGFR Mutations in an Untreated Lung Adenocarcinoma Patient and Response to Erlotinib 
Chapter 23 The Polymerase Chain Reaction
Multiplex Diagnosis of Oncogenic Fusion and MET Exon Skipping by Molecular Counting Using Formalin-Fixed Paraffin Embedded Lung Adenocarcinoma Tissues 
qRT-PCR analysis of expression profile of GbNAC1.
Figure 4 Radiogenomics analysis can reveal relationships
ERCC1 mRNA Expression Is Not Associated with Response and Survival after Platinum-Based Chemotherapy Regimens in Advanced Non-Small Cell Lung Cancer 
Figure 1 Overview of the imaging biomarker roadmap
Hironori Hara et al. BTS 2018;3:
Detection of chlamydia trachomatis DNA in archival paraffinized specimens from chronic salpingitis cases using the polymerase chain reaction  Endrika.
Validation and Reproducibility of a Microarray-Based Gene Expression Test for Tumor Identification in Formalin-Fixed, Paraffin-Embedded Specimens  Raji.
LXs reverse established DKD. (A) Study design overview.
Published in Thyroid. January 2014, 24(1): DOI: /thy
Empiric versus Site-Directed Chemotherapy in Cancer of Unknown Primary
Figure 1. Chloride channel-3 (CIC-3) expression in breast tumor tissues. (A) Chloride channels (ClC-1–7, CFTR) are expressed in human breast cancer tissues.
Pre- and post-vorinostat values of ER-related gene expression using the Oncotype DX 21-gene assay. Pre- and post-vorinostat values of ER-related gene expression.
VEGF expression in neoplastic and normal prostate tissue.
A, TSG-6 staining in TRAMP tissue and TRAMP cell lines.
Cisplatin plus PTUPB decreases proliferation and angiogenesis but increases apoptosis as determined by immunohistochemical (IHC) analysis. Cisplatin plus.
Molecular signatures are independent of tumor stage and grade.
Transcripts enriched and depleted in NB TICs compared with SKPs and other tumor tissues. Transcripts enriched and depleted in NB TICs compared with SKPs.
Immunohistochemical analysis of dUTPase in human breast adenocarcinoma
Figure 3. Immunohistochemistry assay for Mfn2 in each group
Visualization of tumor vasculature using anti-CD31 immunohistochemistry. Visualization of tumor vasculature using anti-CD31 immunohistochemistry. Micrographs.
Expression of S100A11 in bulk pancreatic tissues (pancreatic cancer, n = 22; IPMN, n = 18; nonneoplastic pancreas, n = 22). Expression of S100A11 in bulk.
Northern blot analysis of maspin in cancer cell lines and human tissues. Northern blot analysis of maspin in cancer cell lines and human tissues. A, HMECs.
Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.
Figure 1 Gene-expression quantification methods
Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.
Detection of mutant alleles using ddPCR.
A, Proportion for different in silico predictions at the RNA level.
Defining the eTME genes.
CD36 expression in tissue adjacent and distal to the tumor.
(A) Gram stain of cyst fluid (magnification, ×200).
Presentation transcript:

Figure 3 Size distribution of fixed, paraffin-embedded tissue RNA from 12 tumor specimens Reproduced with permission from Cronin M et al. (2004) Measurement of gene expression in archival paraffin-embedded tissues: development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay. Am J Pathol 164: 35−42 Paik S et al. (2005) Technology Insight: application of molecular techniques to formalin-fixed paraffin-embedded tissues from breast cancer Nat Clin Pract Oncol 2: 246–254 10.1038/ncponc0171