اساس اندازه گیری در سل کانتر

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Presentation transcript:

اساس اندازه گیری در سل کانتر اساس اندازه گیری در سل کانتر

سل کانتر Orphee

Electrolytes solutions allow current to pass from them شرکت سپهر آنالیز Like ISOTON III

www.labservice.ir

A red cell passes through RBC aperture The CBC principle A red cell passes through RBC aperture Red Blood Cell Sensing Zone Oscilloscope Oscilloscope

A red cell passes through RBC aperture The CBC principle A red cell passes through RBC aperture Red Blood Cell Sensing Zone Oscilloscope Oscilloscope

A red cell passes through RBC aperture The CBC principle A red cell passes through RBC aperture Red Blood Cell Sensing Zone Oscilloscope Oscilloscope

A White cell passes through WBC aperture The CBC principle A White cell passes through WBC aperture Neutrophil Oscilloscope Sensing Zone Oscilloscope

A White cell passes through WBC aperture The CBC principle A White cell passes through WBC aperture Neutrophil Oscilloscope Sensing Zone Oscilloscope

A White cell passes through WBC aperture The CBC principle A White cell passes through WBC aperture Neutrophil Oscilloscope Sensing Zone Oscilloscope

Passage of a RBC & Plt through aperture RBCs + - PLT Cross section of RBC aperture (60x50 micron) Oscilloscope screen

Passage of a RBC & Plt through aperture RBCs + - PLT Cross section of RBC aperture (60x50 micron) Oscilloscope screen

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System without sweep flow RBCs recirculating + - RBC PLT Oscilloscope screen Sensing Zone

System with sweep flow + - RBCs pushed away by diluent RBC PLT Oscilloscope screen Sensing Zone Diluent stream

System with sweep flow + - RBCs pushed away by diluent RBC PLT Oscilloscope screen Sensing Zone Diluent stream

System with sweep flow + - RBCs pushed away by diluent RBC PLT Oscilloscope screen Sensing Zone Diluent stream

System with sweep flow + - RBCs pushed away by diluent RBC PLT Oscilloscope screen Sensing Zone Diluent stream

System with sweep flow + - RBCs pushed away by diluent RBC PLT Oscilloscope screen Sensing Zone Diluent stream

Sweep Flow technology RBCs swept away from sensing zone Diluent stream

Pulse editing Cell flows through centre of aperture Good Pulse Diluent stream

Pulse editing Non central cell flow Pulse to be edited Diluent stream

Coincidence Correction Pulse to be edited Diluent stream

Applying Thresholds to separate Plt from RBC 20 fl 2 fl Base line Platelets RBC of various sizes

RBC histogram 256 channel high resolution RBC histogram Main RBC population RBC doublets 256 channel high resolution RBC histogram display cell population data between 24 and 350 fl

PLT histogram and curve fitting A fitted curve accurately reflecting the collected data is displayed between 2 and 35 fl Raw data Fitted Curve displayed up to 35fl PLT histogram is based on 64 channels

PLT curve fitting The Platelet count is derived from the number of cells under the fitted curve beyond the 35fl display limit extending up to 70 fl Raw data Fitted Curve extend up to 70 fl to report very large PLT count

PLT curve fitting The curve fitting process allows more accurate counts when platelets of larger than 20 fl are present

PLT curve fitting The curve fitting process allows more accurate counts when platelets of larger than 20 fl are present PLTs RBCs

CBC WBC histogram 256 channel high resolution WBC histogram Lymphocyte Monocyte Granulocyte 256 channel high resolution WBC histogram display cell population data between 35 and 450 fl

Coulter WBC histogram Mono Lymp Neut Baso Eos

Hgb Measurement Derivation Signal ADC Processor WBC Bath HGB Lamp Absorbance= log 10 ( VR / Vs ) Where VR = Reference voltage Vs = Sample voltage HGB Sensor & PRE-AMP Sample