Outbreak of immediate-type hydrolyzed wheat protein allergy due to a facial soap in Japan Akiko Yagami, MD, PhD, Michiko Aihara, MD, PhD, Zenro Ikezawa,

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Outbreak of immediate-type hydrolyzed wheat protein allergy due to a facial soap in Japan Akiko Yagami, MD, PhD, Michiko Aihara, MD, PhD, Zenro Ikezawa, MD, PhD, Michihiro Hide, MD, PhD, Reiko Kishikawa, MD, PhD, Eishin Morita, MD, PhD, Yuko Chinuki, MD, PhD, Yuma Fukutomi, MD, PhD, Atsuo Urisu, MD, PhD, Atsuki Fukushima, MD, PhD, Yasuharu Itagaki, PhD, Shin-ichi Sugiura, PhD, Hiroyuki Tanaka, PhD, Reiko Teshima, PhD, Zenichiro Kato, MD, PhD, Emiko Noguchi, MD, PhD, Masashi Nakamura, PhD, Hirohisa Saito, MD, PhD, Kayoko Matsunaga, MD, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 3, Pages 879-881.e7 (September 2017) DOI: 10.1016/j.jaci.2017.03.019 Copyright © 2017 The Authors Terms and Conditions

Fig 1 The number of patients registered per month and cumulative total number between 2012 and 2014. Journal of Allergy and Clinical Immunology 2017 140, 879-881.e7DOI: (10.1016/j.jaci.2017.03.019) Copyright © 2017 The Authors Terms and Conditions

Fig E1 Flowchart of the patients' registration for immediate-type wheat allergy caused by a specific HWP (HWP-IWA). Journal of Allergy and Clinical Immunology 2017 140, 879-881.e7DOI: (10.1016/j.jaci.2017.03.019) Copyright © 2017 The Authors Terms and Conditions

Fig E2 Age and sex distribution of the Japanese patients allergic to HWP in facial soaps. Journal of Allergy and Clinical Immunology 2017 140, 879-881.e7DOI: (10.1016/j.jaci.2017.03.019) Copyright © 2017 The Authors Terms and Conditions

Fig E3 IgE antibody levels against Glupearl 19S. A, SDS-PAGE image of Glupearl 19S and gluten. M: molecular weight marker; lane 1: Glupearl 19S, lane 2: gluten. B, Dot plots of serum Glupearl 19S specific IgE levels were quantified by ELISA using sera from patients with conventional WDEIA (CO-WDEIA, n = 7), HWP-IWA who satisfied the diagnostic criteria described in Table E2 (HWP, n = 20), subjects who had used the soaps but who did not satisfy diagnostic criteria (HWP-Negative, n = 20), and healthy controls without wheat allergy (Healthy, n = 7). Units greater than 100 are described as 100. Dashed line indicates a cutoff value (5 units). Journal of Allergy and Clinical Immunology 2017 140, 879-881.e7DOI: (10.1016/j.jaci.2017.03.019) Copyright © 2017 The Authors Terms and Conditions