Volume 117, Issue 1, Pages 73-81 (July 1999) Inflammatory alterations in mesenteric adipose tissue in Crohn's disease  Pierre Desreumaux*,‡, Olivier Ernst§, Karel Geboes∥, Luc Gambiez¶, Dominique Berrebi#, Heide Müller-Alouf‡, Samira Hafraoui*, Dominique Emilie**, Nadine Ectors∥, Michel Peuchmaur#, Antoine Cortot*, Monique Capron‡, Johan Auwerx‡‡, Jean-Frédéric Colombel*  Gastroenterology  Volume 117, Issue 1, Pages 73-81 (July 1999) DOI: 10.1016/S0016-5085(99)70552-4 Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 1 Areas of intra-abdominal and abdominal subcutaneous fat tissues in an MRI cross-sectional area centered on the umbilicus in a patient with CD and a healthy control. CD patients and controls had similar areas (cm2) of total abdominal fat. The ratio of intra-abdominal fat area to total abdominal fat area was significantly higher in patients with CD than in controls (c)(*P = 0.012). Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 2 Individual values and means (horizontal bar) of PPARγ cDNA molecules per molecule of β-actin cDNA in the subcutaneous and mesenteric tissues of patients with CD contiguous to involved intestine (CDi) or healthy intestine (CDh) and in controls (C). *P < 0.01, significant difference between the PPARγ cDNA molecules in the mesenteric tissues of patients with CD compared with the mesenteric tissue of controls. Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 3 Individual values and means (horizontal bar) of TNF-α cDNA molecules per molecule of β-actin cDNA in the mesenteric tissues of 9 patients with CD contiguous to involved intestine (CDi) or healthy intestine (CDh) and in PBMCs stimulated by lipopolysaccharide isolated from 7 patients with CD (C). Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 4 Representative TNF-α immunostaining in mononuclear cells (arrows) located near the peritoneal surface of the mesenteric tissue contiguous to involved intestine in a patient with CD (indirect immunoperoxidase; original magnification 400×). Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 5 Positive immunostaining using an antibody directed against TNF-α in adipocytes of the mesenteric WAT in a patient with CD. The staining appeared as a dark brown rim of cytoplasm in the periphery of the cell (indirect immunoperoxidase; original magnification 1000×). Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 6 In situ hybridization with TNF-α mRNA antisense probe on mesenteric WAT performed in a patient with CD. All adipocytes of the mesenteric specimen of patients with CD were stained (original magnification 1000×). Inset, no labeling was observed on the semiserial section of the same specimen hybridized with the sense control probe (original magnification 1000×). Gastroenterology 1999 117, 73-81DOI: (10.1016/S0016-5085(99)70552-4) Copyright © 1999 American Gastroenterological Association Terms and Conditions