What tools do scientists use to test the toxicity of different chemicals?
Outline Bioassay background (5 mins) Activity 1: Sublethal effects of chemicals (15-20 mins) Activity 2: Lethal effects of chemicals (15-20 mins) Discussion (5-10 mins)
Teaching Standards Environmental science standards Biology standards Recognize that humans are part of the global ecosystem and evaluate the effects of human activities on ecosystems Biology standards Assess how human activities such as pesticide use influence and modify the environment Relate animal adaptations, including behaviors, to the ability to survive stressful environmental conditions
Bioassays What is a bioassay? What is a bioindicator? What characteristics make a good bioindicator species? Sensitivity Representative of the system you are interested in?
Bioindicators
Daphnia Crustaceans- “water fleas” Filter feeders Up to 1/4th of an inch in length-easily observable under dissecting microscope Nearly translucent carapace Sensitive to environmental changes heart beat video
Acute vs Chronic effect Sublethal effects Once we choose an indicator species, how do we measure the effects of a chemical? Acute vs Chronic effect Sublethal effects Behavior Heart rate-stimulants vs. depressants Lethal effects LD50 Sublethal effects Fig 1. Effect of two insecticides on insect survival
Dose/concentration is important
Activity 1 Record baseline heart rate Sublethal effects of chemicals on Daphnia magna Record baseline heart rate Add a single drop of each solution and measure change in heart rate Ethanol, sugar solution, hot and cold water
Activity 1 Questions Which solutions showed an increase in heart rate? Which solutions showed a decrease in heart rate? Which solutions would you classify as stimulants? Depressants?
Lethal effects of chemicals on Daphnia magna Activity 2 Lethal effects of chemicals on Daphnia magna
Activity 2 Questions What toxicant did you choose to test? Why? Which toxicant concentration appeared to have little effect on the Daphnia? Can you use the results of your bioassay to make predictions about the effect of this toxicant on humans?