Laboratory Biosafety and Biosecurity Assist. Prof. Dr. Majed H. Mohammed Ph.D. Virology and Molecular Cell Biology Scientific Research Center

Topics to be discussed What is biosafety and biosecurity Why we need Levels of biosafety Biosafety cabinet types

BIOSAFETY: Preventing lab-acquired infections Bacteria Viruses Fungi Human blood, unfixed tissue Human cell lines Recombinant DNA

Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community { CDC }

Why we need biosafety ???? Lab has hazards of processing infectious agents Accidental threat to workers and environment To have adherence with safety regulations while dealing with highly infectious agents

Levels of Containment BL1 - microorganisms that don’t consistently cause disease in healthy adults E. coli Basic laboratory Standard Microbiological Practices

Biosafety Level 2 (BSL-2) Agents associated with human disease Agents associated with human disease Agents include measles virus, Salmonella species, pathogenic Toxoplasma, Clostridium botulinum, hepatitis B virus

Levels of Containment M. tuberculosis, yellow fever virus, hantavirus, BL3 - microorganisms that cause serious disease, transmitted by inhalation M. tuberculosis, yellow fever virus, hantavirus,

Levels of Containment Ebola virus, Marburg virus BL4 - microorganisms that cause lethal disease, with no known treatment or vaccine Ebola virus, Marburg virus Maximum containment lab; positive pressure ventilated suits (moon suits)

BSL 4

biosafety cabinet

WORKING SAFELY IN A BSC Before using the cabinet: Ensure BSC is certified Turn off UV lamp; turn on fluorescent lamp Disinfect work surfaces with appropriate disinfectant Place essential items inside cabinet Allow the blower to run for 5-10 min before work

WORKING SAFELY IN A BSC After using the cabinet: Leave blower on at least 5 minutes to purge cabinet Remove and decontaminate equipment and materials Disinfect cabinet surfaces Turn off blower and fluorescent lamp, turn on UV lamp

Introduction Biosafety cabinets (BSCs) are primary means of containment, developed for working safely with infectious micro-organisms

Hepa filter HEPA – High efficiency particulate air filter It removes the most penetrating particle size (MPPS) of 0.3 μm with an efficiency of at least 99.97 %

http://en.wikipedia.org/wiki/HEPA

Class II BSC Class II Type A1 Class II BSC

THANK YOU! ANY QUESTIONS?