Volume 33, Pages 174-180 (January 2017) TLR and NLRP3 inflammasome expression deregulation in macrophages of adult rats subjected to neonatal malnutrition and infected with methicillin-resistant Staphylococcus aureus  Natália Gomes de Morais, Ph.D., Thacianna Barreto da Costa, Ph.D., Joana Maria Bezerra de Lira, B.A., Suênia da Cunha Gonçalves de Albuquerque, Valéria Rêgo Alves Pereira, Ph.D., Milena de Paiva Cavalcanti, Ph.D., Célia Maria Machado Barbosa de Castro, Ph.D.  Nutrition  Volume 33, Pages 174-180 (January 2017) DOI: 10.1016/j.nut.2016.06.005 Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 1 Weight curve evolution during the neonatal malnutrition (21 d) and nutritional supplementation (23–90 d) in the groups (N = nourished and M = malnourished). Differences between N and M groups were considered significant at P < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 2 Plot of cDNA amplification curves of the iNOS (lipopolysaccharide) positive control (at the ratio of 1 fg to 1 ng). Threshold is the detection threshold. Ct, cycle threshold; iNOS, inducible nitric oxide synthase; NTC, negative control of reaction; TLR, toll-like receptor. Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 3 Relative TLR-2 receptor expression in the groups (N = nourished; M = malnourished) and systems. MSSA, methicillin-sensitive Staphylococcus aureus; MRSA, methicillin-resistant Staphylococcus aureus; PC, positive control; TLR, toll-like receptor. Differences between N and M groups were considered significant at P < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 4 Relative TLR-4 receptor expression in the groups (N = nourished; M = malnourished) and systems. MSSA, methicillin-sensitive Staphylococcus aureus; MRSA, methicillin-resistant Staphylococcus aureus; PC, positive control; TLR, toll-like receptor. Differences between N and M groups were considered significant at P < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 5 Relative NLRP3 receptor expression in the groups (N = nourished; M = malnourished) and systems. MSSA, methicillin-sensitive Staphylococcus aureus; MRSA, methicillin-resistant Staphylococcus aureus; PC, positive control; TLR, toll-like receptor. Differences between N and M groups were considered significant at P < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 6 Relative caspase-1 enzyme expression in the groups (N = nourished; M = malnourished) and systems. MSSA, methicillin-sensitive Staphylococcus aureus; MRSA, methicillin-resistant Staphylococcus aureus; PC, positive control. Differences between N and M groups were considered significant at P < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 7 IL-1 β levels in the supernatant of alveolar macrophage cultures in the groups (N = nourished; M = malnourished) and systems. C, negative control; IL, interleukin; MSSA, methicillin-sensitive Staphylococcus aureus; MRSA, methicillin-resistant Staphylococcus aureus; PC, positive control. Differences between N and M groups were considered significant at p < 0.05 and were represented by the symbol (*). Nutrition 2017 33, 174-180DOI: (10.1016/j.nut.2016.06.005) Copyright © 2016 Elsevier Inc. Terms and Conditions