The Microscope

Types of microscopes The compound light microscope The Dissecting microscope The electron microscope

The microscope used most commonly in cell study For study with this microscope, the specimen must be thin enough for light to pass through it easily

Compound Light microscope It has 2 lenses Light passes through a specimen, then passes through the objective lens The enlarged image is magnified AGAIN by the eyepiece lens

The dissecting microscope allows you to see large specimens in three-dimensional quality The electron microscope produces magnification of more than 100,000 times

Parts and functions The tube that connects the oculars(the eyepiece) to the revolving nose piece which holds objectives Body tube

the lens on the top of the microscope that you look through Eyepiece (ocular lens)

Low-power objective: The shorter objective{yellow} Contains lenses that magnify objects 10 times

the low power lens is more zoomed out and you can see a larger area so you see more of your image

High- power objective: The longer objective{blue} Contains lenses that usually magnify 40 times

When viewing under the high power objective {the blue one}, you can see better details of the image, but you cannot see as much of the image

Coarse adjustment the larger knob on the microscope When you move this knob, it moves the stage up or down

Fine adjustment This is the small knob on the microscope The fine adjustment sharpens the image {makes you see clearer} under low power

Stage: Stage clips: platform for holding the slides holds the slide on the stage

used for carrying the microscope Arm and Base

Nosepiece This rotates the objectives

this controls the amount of light passing through a specimen The diaphragm

The circle of light you see when you look through the microscope Field of view

Microscope “RULES” Never use the coarse adjustment under high power because you can break the slide

What is the formula for total magnification? To determine the total magnification, you multiply the eyepiece by the objective lens Total magnification refers to the total amount that the image is enlarged

Under the low power objective, the field of view gets larger Under the high power objective, the field of view gets smaller

How does an image appear under the microscope? The final image appears enlarged, upside down and backward