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Presentation transcript:

(optional - but then again, all of these are optional) Advanced Exercises (optional - but then again, all of these are optional)

Exercise 1 I posted solution to all the previous exercises. Go through my solutions and compare to yours. Experiment with some of the alternatives I show. Let me know of any mistakes. (I know there’s at least one.)

#2, FASTA files I put a FASTA file at /coursehome/dalke/ls_orchid.fasta (It’s part of the Biopython regression suite.) Take a look at the file using more (or less). Can you figure out the file format?

FASTA format A FASTA file contains 0 or more records Each record starts with a header line The first character of the header is a “>” After that are a bunch of sequence lines After the sequence lines is a blanks line (In real FASTA files that line is optional. Don’t worry about that, since this version is easier to parse.)

Write a program to count the number of records in a FASTA file. Exercise #3 Write a program to count the number of records in a FASTA file. Once done, modify that program to print the total number of bases present and the total count of each base found.

Exercise #4 Write a program to print only the header lines in that FASTA file. It must not print the leading “>”.

Exercise #5 Write a program to find the record(s) where the header line contains the text P.tonsum. Print it out, in FASTA format. (That is, the output should not be changed.)

Command-line arguments In Python you can get the list of command-line arguments with sys.argv. This is a normal Python list. > cat print_args.py import sys print "Command-line arguments are:", sys.argv > python print_args.py Command-line arguments are: ['print_args.py'] > python print_args.py ls_orchid.fasta Command-line arguments are: ['print_args.py', 'ls_orchid.fasta'] > python print_args.py *.seq Command-line arguments are: ['print_args.py', '10_sequences.seq', 'ambiguous_sequences.seq', 'many_sequences.seq', 'sequences.seq'] >

Exercise #6 Write a program which gets two command line arguments, turns them into floats, and prints the sum. Note that sys.argv[0] is always the name of the Python program so you’ll need to use [1] and [2] You can find this program in the worked out solutions, under the first Monday. Compare it to yours.

Exercise #7 Modify your solution from #5 so that it uses the first user argument (sys.argv[0]) as the text to search for in the header. Have it print all FASTA records which contain that text. A good test case is “P.”

Exercise #8 Write a program to read a FASTA file and print the header line (without the leading “>”) if and only if the sequence ends with a C. Use a command-line argument to get the filename. Test it against both FASTA files in my directory.