Periodic elevation of regulatory T cells on the day of embryo transfer is associated with better in vitro fertilization outcome Wen-Juan Wang, Fu-Jun.

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Periodic elevation of regulatory T cells on the day of embryo transfer is associated with better in vitro fertilization outcome Wen-Juan Wang, Fu-Jun Liu, Xia Zhang, Xue-Mei Liu, Qing-Lan Qu, Feng-Hua Li, Li-Li Zhuang, Xiao-Xiao Li, Cui-Fang Hao Journal of Reproductive Immunology Volume 119, Pages 49-53 (February 2017) DOI: 10.1016/j.jri.2017.01.002 Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 1 Pregnant women had a significantly higher percentage of CD4+CD25+CD127dim/− Tregs in CD4+ T cells than non-pregnant women on the day of ET (P<0.05) and at 14days after ET (P<0.05) used non-parametric Mann-Whitney U test, there is no difference between the pregnant and non-pregnant women at 5days after ET (5.4±0.2 vs 5.4±0.2%, P>0.05). No obvious variations were observed in the Treg levels in non-pregnant women. Box and whisker plots: bar (horizontal line)=median; box=25th and 75th percentiles; whiskers=extend to the extreme values. Journal of Reproductive Immunology 2017 119, 49-53DOI: (10.1016/j.jri.2017.01.002) Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 2 The serum IL-10 and TGF-β levels were significantly higher in the pregnant patients than in the non-pregnant patients (P<0.03 and P<0.02 respectively) at 14days after ET (n=10) used non-parametric Mann-Whitney U test. Box and whisker plots: bar (horizontal line)=median; box=25th and 75th percentiles; whiskers=extend to the extreme values. Journal of Reproductive Immunology 2017 119, 49-53DOI: (10.1016/j.jri.2017.01.002) Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 3 Fold of isolated CD4+CD25+CD127dim/− cell expansion after 7days of culture. The pregnant group had a higher fold of Treg expansion than the non-pregnant group (P<0.05) used non-parametric Mann-Whitney U test. Values presented are from 10 independent experiments (n=10). The longer bar (horizontal line)=median; two shorter bar=25th and 75th percentiles. Journal of Reproductive Immunology 2017 119, 49-53DOI: (10.1016/j.jri.2017.01.002) Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 4 Purity of CD4+CD25+CD127dim/− cells after 7days of culture in the two groups (n=10). The percentage of CD4+CD25+CD127dim/− cells did not significantly differ between the two groups (P>0.05) used non-parametric Mann-Whitney U test. The longer bar (horizontal line)=median; two shorter bar=25th and 75th percentiles. Journal of Reproductive Immunology 2017 119, 49-53DOI: (10.1016/j.jri.2017.01.002) Copyright © 2017 Elsevier B.V. Terms and Conditions