The novel exercise-induced hormone irisin protects against neuronal injury via activation of the Akt and ERK1/2 signaling pathways and contributes to.

Slides:



Advertisements
Similar presentations
Presented by R. Sruthi PhD Scholar Department of Pharmacy Under Supervision of Dr. Rajeev Taliyan Assistant Professor Department of Pharmacy.
Advertisements

Supervisor: Yonggeun Hong (DVM, PhD)
Tetrahydrocurcumin provides neuroprotection in rats after traumatic brain injury: autophagy and the PI3K/AKT pathways as a potential mechanism  Yongyue.
Fig. 2. Neuroprotective effects of ex-4 through the G protein-coupled GLP-1 receptor. (A) Infarct volume was assessed by TTC staining after tMCAO in rats.
PGE2 receptor agonist misoprostol protects brain against intracerebral hemorrhage in mice  He Wu, Tao Wu, Wei Hua, Xianghui Dong, Yufeng Gao, Xiaochun.
Berberine lowers blood glucose in type 2 diabetes mellitus patients through increasing insulin receptor expression  Hao Zhang, Jing Wei, Rong Xue, Jin-Dan.
Tumor necrosis factor α is associated with insulin-mediated suppression of free fatty acids and net lipid oxidation in HIV-infected patients with lipodystrophy 
Advisor: Yonggeun Hong (DVM, PhD)
Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-kB Signaling Pathway Cell Physiol Biochem 2017;43:481–491 - DOI: /
Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to.
Α-Lipoic Acid Promotes Neurological Recovery After Ischemic Stroke by Activating the Nrf2/HO-1 Pathway to Attenuate Oxidative Damage Cell Physiol Biochem.
Cerebrovasc Dis 2016;42: DOI: /
Contribution of nitric oxide synthase (NOS) in blood–brain barrier disruption during acute focal cerebral ischemia in normal rat  Mohammad T. Mohammadi,
Volume 4, Issue 1, Pages (July 2006)
Vascular PPARδ Protects Against Stroke-Induced Brain Injury
Volume 78, Issue 3, Pages (August 2010)
Dexmedetomidine, an α-2a adrenergic agonist, promotes ischemic tolerance in a murine model of spinal cord ischemia-reperfusion  Marshall T. Bell, MD,
ERRγ—A New Player in β Cell Maturation
Bugs and Brain: How Infection Makes You Feel Blue
Inhibition of the MEK/ERK pathway reduces microglial activation and interleukin-1-beta expression in spinal cord ischemia/reperfusion injury in rats 
Cerebral Mast Cells Participate In Postoperative Cognitive Dysfunction by Promoting Astrocyte Activation Cell Physiol Biochem 2016;40: DOI: /
Volume 45, Issue 5, Pages (November 2016)
Safia Costes, Peter C. Butler  Cell Metabolism 
Volume 137, Issue 6, Pages e2 (December 2009)
Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,
Baiya Krishnadasan, MD, Babu V
Muscle Over Mind Cell Metabolism
Burning Fat and Building Bone by FSH Blockade
Burning Fat and Building Bone by FSH Blockade
J.R. Liu, C. Baek, X.H. Han, P. Shoureshi, S.G. Soriano 
Tetramethylpyrazine protects spinal cord and reduces inflammation in a rat model of spinal cord ischemia-reperfusion injury  Lihong Fan, MD, Kunzheng.
Volume 15, Issue 3, Pages (March 2012)
Volume 86, Issue 2, Pages (August 2014)
L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong 
Volume 17, Issue 5, Pages (May 2013)
The essential role of endothelial nitric oxide synthase activation in insulin-mediated neuroprotection against ischemic stroke in diabetes  Shiang-Suo.
Mesenchymal Stem Cells Overexpressing Interleukin-10 Promote Neuroprotection in Experimental Acute Ischemic Stroke  Masataka Nakajima, Chikako Nito, Kota.
Irisin, Turning Up the Heat
A novel NF-κB inhibitor, dehydroxymethylepoxyquinomicin, ameliorates inflammatory colonic injury in mice  Tohru Funakoshi, Kenichiro Yamashita, Nobuki.
Volume 117, Issue 4, Pages (October 1999)
Enhanced expression of glucose transporter-1 in vascular smooth muscle cells via the Akt/tuberous sclerosis complex subunit 2 (TSC2)/mammalian target.
Stuart J. Mills, Jason J. Ashworth, Stephen C. Gilliver, Matthew J
Volume 79, Issue 1, Pages (January 2011)
Volume 26, Issue 1, Pages (March 2019)
Macrophages Contribute to the Progression of Infantile Hemangioma by Regulating the Proliferation and Differentiation of Hemangioma Stem Cells  Wei Zhang,
Bin Gao, Hua Wang, Fouad Lafdil, Dechun Feng  Journal of Hepatology 
Volume 27, Issue 2, Pages (August 2007)
Ribosomal Protein S3 Gene Silencing Protects Against Cigarette Smoke-Induced Acute Lung Injury  Jinrui Dong, Wupeng Liao, Hong Yong Peh, W.S. Daniel Tan,
Volume 119, Issue 4, Pages (October 2000)
Muscle Over Mind Cell Metabolism
Volume 12, Issue 1, Pages (July 2015)
Deletion of the epidermal growth factor receptor in renal proximal tubule epithelial cells delays recovery from acute kidney injury  Jianchun Chen, Jian-Kang.
All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes 
Hong T. Hua, MD, Hassan Al-Badawi, MD, Fateh Entabi, MD, Michael C
Banking on ATM as a new target in metabolic syndrome
Volume 15, Issue 3, Pages (March 2012)
Chi-Hyun Park, Youngji Moon, Chung Min Shin, Jin Ho Chung 
Toll-like receptor 4 mediates ischemia/reperfusion injury of the heart
CD4+ T lymphocytes mediate acute pulmonary ischemia–reperfusion injury
Neuroprotection from Stroke in the Absence of MHCI or PirB
Volume 8, Issue 4, Pages (October 2005)
Volume 160, Issue 1, Pages (January 2015)
Volume 40, Issue 4, Pages (April 2014)
Sevoflurane preconditioning-induced neuroprotection is associated with Akt activation via carboxy-terminal modulator protein inhibition  Y. Chen, H. Nie,
Ann Marie Schmidt, Kathryn J. Moore  Cell Metabolism 
Volume 137, Issue 6, Pages e2 (December 2009)
BDNF (I)rising from Exercise
Nicotinamide Mononucleotide, a Key NAD+ Intermediate, Treats the Pathophysiology of Diet- and Age-Induced Diabetes in Mice  Jun Yoshino, Kathryn F. Mills,
Resveratrol neuroprotective effects during focal cerebral ischemia injury via nitric oxide mechanism in rats  Shen-Kou Tsai, MD, PhD, Li-Man Hung, PhD,
Volume 26, Issue 1, Pages 1-10.e7 (January 2019)
Presentation transcript:

The novel exercise-induced hormone irisin protects against neuronal injury via activation of the Akt and ERK1/2 signaling pathways and contributes to the neuroprotection of physical exercise in cerebral ischemia  Dong-Jie Li, Yong-Hua Li, Hong-Bin Yuan, Le-Feng Qu, Pei Wang  Metabolism - Clinical and Experimental  Volume 68, Pages 31-42 (March 2017) DOI: 10.1016/j.metabol.2016.12.003 Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 1 Changes of plasma irisin level and intramuscular irisin precursor FNDC5 expression after cerebral ischemic stroke. (A) Comparison of circulating irisin levels between Sham-operated and MCAO mice at 24h after surgery. *P<0.01. N=6 per group. (B) Comparison of irisin precursor FNDC5 protein expression in skeletal muscle between Sham-operated and MCAO mice at 24h after surgery. *P<0.01. N=6 per group. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 2 Correlations between plasma irisin levels and brain infarction volume, neurological deficit score and plasma pro-inflammatory factors. (A) Correlation between plasma irisin levels and brain infarct volume. (B) Correlations between plasma irisin levels and neurological deficit scores. (C) Correlation between plasma irisin levels and plasma TNF-α levels. (D) Correlation between plasma irisin levels and plasma IL-6 levels. (E) Correlation between plasma irisin levels and plasma IL-1β levels. N=24 in A-E. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 3 Effects of irisin treatment on neuronal injury in MCAO mice. (A) The experimental design for the evaluation of action of irisin in MCAO model. (B) Effect of irisin on brain infarct volume. (C-D) Beam walking test and grip test in MCAO mice. (E) Effect of irisin on the change of body weight. (F) Effect of irisin on the brain edema. *P<0.05 versus sham, #P<0.05 versus vehicle. n=6–8 for each group. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 4 Effects of irisin treatment on post-ischemic neuroinflammation in MCAO mice. (A-B) Immunohistochemistry staining of Iba-1 (A) and MPO-1 (B) in peri-infarct brain tissues of MCAO mice. (C-D) ELISA analyses of TNF-α and IL-6 mRNA expression in peri-infarct brain tissues of MCAO mice. *P<0.05 versus vehicle. n=6–8 for each group. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 5 Effects of irisin treatment on oxidative stress in MCAO mice. (A) Effects of irisin treatment on NO2− Tyr level in peri-infarct brain tissues of MCAO mice. (B) Effects of irisin treatment on superoxide anion level in peri-infarct brain tissues of MCAO mice. (C) Effects of irisin treatment on superoxide anion level in peri-infarct brain tissues of MCAO mice. (D) Effects of irisin treatment on MDA level in peri-infarct brain tissues of MCAO mice. *P<0.05 versus sham, #P<0.05 versus vehicle. n=6–8 for each group. NS, no significance. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 6 Involvement of ERK1/2 and Akt signaling pathways in the neuroprotection of irisin. (A) Immunoblotting analyses showing the phosphorylation of ERK1/2 and Akt signaling pathways by irisin in brain tissues of MCAO mice. *P<0.05 versus vehicle. n=4 for each group. (B-D) Blockade of ERK1/2 and Akt signaling pathways by specific chemical inhibitors partly abolished the beneficial action of irisin on neurological function (B) and neuroinflammation (C-D). *P<0.05 versus blank, #P<0.05 versus irisin treatment. n=6. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 7 Irisin contributes to the neuroprotection of physical exercise against ischemic stroke. (A) The experimental design for the investigation on the role of irisin in the neuroprotection of physical exercise. (B) Effect of administration of irisin neutralizing antibody (irisin NA) on the brain infarct volume. *P<0.05 versus sedentary, #P<0.05 versus control IgG. n=6. (C) Effect of administration of irisin NA on the neurological function. *P<0.05 versus sedentary, #P<0.05 versus control IgG. n=6. (D-E) Effect of administration of irisin NA on the TNF-α (D) and IL-6 (E) mRNA levels. *P<0.05 versus sedentary, #P<0.05 versus control IgG. n=6. Metabolism - Clinical and Experimental 2017 68, 31-42DOI: (10.1016/j.metabol.2016.12.003) Copyright © 2016 Elsevier Inc. Terms and Conditions