TUDA Progress

TUDA Sample Collection Cohort Completed bloods and questionnaires Data entry Completed Dexa scans Cognitive 1465 1360 (not double) Bone 771 635 641 Hypertension 1800 512 1749

TUDA Laboratory Analysis Blood Metabolite Cognitive (N=1465) Completed Results Bone (N= 771) Hypertensive (N=1800) Serum folate 1286 564 1764 Red cell folate Serum total B12 1670 Serum holoTC 900 100 Plasma tHcy 1300 500 Plasma MMA Plasma B6 (PLP) 1200 200 1400 Red cell B2 (EGRAC) 1350 Serum D vitamers 1100 Serum Cross Laps N/A 1750 Serum Osteocalcin Serum BAP Plasma Bone TRAP

TUDA DNA Extractions Cognitive (N=1385 ) DNA extracted Bone (N= 641) Hypertensive (N= ) 1073 800 Prepared DNA is stored in two tubes; Tube 1 contains 100 µL of normalised DNA at a concentration of approximately 100 ng/µl. Tube 2 contains the original purified stock DNA at variable concentrations.

Laboratory Analysis for NANS (N=1145) Blood Metabolite Final Results Serum folate 1125 Red cell folate 1129 Serum total B12 1124 Plasma tHcy 1130 Red cell B2 (EGRAC) 400 Buffy coat for DNA Extracted DNA 1127 Lower numbers than 1145 for each metabolite are due to missing serum, plasma or buffy coat samples that were not included in the shipments from UCD/UUC