Identification of necrophagous fly species using ISSR and SCAR markers

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Identification of necrophagous fly species using ISSR and SCAR markers Lin He, Sibao Wang, Xuexia Miao, Hong Wu, Yongping Huang Forensic Science International Volume 168, Issue 2, Pages 148-153 (May 2007) DOI: 10.1016/j.forsciint.2006.07.006 Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Amplification of genetic DNA in five necrophagous fly species using primer (CA)6GT. 1, 2: Lucilia sericata, adult; 3, 4: L. sericata, second instar larva; 5, 6: L. sericata, third instar larva; 7, 8: Aldrichina grahami, adult; 9, 10: Chrysomya megacephala, adult; 11, 12: C. megacephala, third instar larva; 13, 14: Musca domestica, adult; 15, 16: Parasarcophaga crassipalpis, adult. Forensic Science International 2007 168, 148-153DOI: (10.1016/j.forsciint.2006.07.006) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Amplification of different individuals using primer (CA)6GT. 1, 3, 5, 7, 9, 11, 13, 15: L. sericata, second instar larva; 2, 4, 6, 8, 10, 12, 14, 16: L. sericata, third instar larva. Forensic Science International 2007 168, 148-153DOI: (10.1016/j.forsciint.2006.07.006) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Agarose gel electrophoresis of the amplification products obtained from species-specific SCAR markers; 1, 2 L. sericata, adult; 3, 4: A. grahami, adult; 5, 6: C. megacephala, adult; 7, 8: P. crassipalpis, adult; 9, 10: M. domestica, adult (molecular size markers (lane M) in base pairs are indicated on the left of each panel. (A) shows the SCAR marker for C. megacephala, (B) shows the SCAR marker for P. crassipalpis and (C) shows the SCAR marker for M. domestica). Forensic Science International 2007 168, 148-153DOI: (10.1016/j.forsciint.2006.07.006) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Reliability test of SCAR marker for M. domestica in three populations collected in Shanghai. The three locations are about 30km from each other. Letters with number in the figure showed the different individual. Forensic Science International 2007 168, 148-153DOI: (10.1016/j.forsciint.2006.07.006) Copyright © 2006 Elsevier Ireland Ltd Terms and Conditions