Scanning Electron Microscopy Laboratory

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Scanning Electron Microscopy (SEM)

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Scanning Electron Microscopy Laboratory A member lab of the HRIF: Kent T. Keyser, Ph.D., Director Scanning Electron Microscope Compositional Imaging (Backscatter Electron, BSE) FEI Quanta 650 FEG (SEM) The SEM in the laboratory features a high resolution field emission gun with environmental technology. It is one of the most versatile SEM instruments available. In this mode, regions with elements of higher atomic number show higher intensity in the image Image: Ceramic Image: Cell High Vacuum Wet STEM Wet scanning-transmission electron microscopy (STEM) is a technique that allows high-resolution transmission imaging of biological samples in a hydrated state, with minimal sample preparation. The traditional SEM method. In high vacuum mode it is possible to obtain surface images of dry, conductive samples with nanometer resolution Image: Porous Manganese Carbonate Templates (Cover of JMC_B) Courtesy of Dr. Kharlampieva Other Beam Deceleration Stereo Imaging Topography Etc… Low Vacuum This is a variable pressure mode, allowing for imaging of non-conductive, dry samples. Image: Mouse Intestine Environmental SEM (ESEM) This is a mode which can be used to image wet, living, and unfixed samples Image: Sea Urchin Image Processing Techniques Consult with William for your image processing needs. Examples of image processing work done: Porosity metrics Cell/Grain Size Dendrite Arm Spacing Graphite Spacing Application specific image montages Various post processing algorithms for HRIF Colorization Energy Dispersive Spectroscopy (EDS) EDS provides a qualitative elemental analysis of samples. This is available for all pressure modes Image: Pyrite Contact Information William Monroe SEM Core Manager 934-8427(office) wsmonroe@uab.edu http://www.uab.edu/scanningelectronmicroscopy/ The SEM Lab is Supported by: High Resolution Imaging Core Department of Materials Science & Engineering College of Arts and Sciences Center for Nanoscale Materials and Biointegration The Office of the Vice President for Research