VIABILITY ASSAYS

What is Viability and Viability assays ?

How we check viability? Tetrazolium assay Lactate dehydrogenase determination Adenylate energy charge Rate of protein or nucleic acid synthesis Colony forming assay

Tetrazolium assay Colometric based Used tetrazolium dye like MTT [3-(4,5-dim ethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] Lactate dehydrogenase Enzyme based Measure by spectrophotomectric assay

Pyruvate +NADH+H+ LDH lactate + NAD+​ Index based , Pyruvate +NADH+H+ LDH lactate + NAD+​ ​Adenylate energy charge Index based The energy charge= ATP+ 0.5ADP ​ ATP+ADP+AMP Interconversion of 3 adenylate AMP ADP ATP ​

Rate of protein or nuclic acid synthesis Rate of protien or DNA synthesis Radioactive amino acids or nucleotides Measure by tritiated thymidine Colony forming assay Viable cells foam colony