Schematic outline of MeDIP

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Schematic outline of MeDIP Schematic outline of MeDIP. Genomic DNA is sheared into approximately 400 to 700 bp using sonication and subsequently denatured. Incubation in 5-mC antibodies, along with standard immunoprecipitation (IP), enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to 1 of 2 DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question. (From http://en.wikipedia.org/wiki/Methylated_DNA_immunoprecipitation.) Source: Methods of Molecular Analysis, The Basic Science of Oncology, 5e Citation: Tannock IF, Hill RP, Bristow RG, Harrington L. The Basic Science of Oncology, 5e; 2016 Available at: http://hemonc.mhmedical.com/DownloadImage.aspx?image=/data/books/1791/tanbas5_ach2_f021.png&sec=124303196&BookID=1791&ChapterSecID=124303110&imagename= Accessed: October 27, 2017 Copyright © 2017 McGraw-Hill Education. All rights reserved