DIRECTIONS to follow ASAP: Read pages 830-831 silently On a new sheet of paper, write the title “Proper Use of the Compound Light Microscope” Write a PHRASE summarizing each of the 10 steps listed

Mind Stretcher 1. (Tell me one experience you have had with a microscope). 2. (List three things you know about cells. Can be anything……)

Spooky Microscope Trivia

Spooky Cells 1 My composition is white and hard, you can find me in an old graveyard.

Microscope Basics

Where you View the Specimen! Ocular lens (Eyepiece) Focuses ocular lens Body Tube Rotates Objective Lenses for Viewing Nosepiece Arm Objectives Each has Different Magnification! Stage Hold Slide in Place Stage Clips Coarse Adjustment Use for Broad Scanning on Low Power Objectives! Regulates Amount of Light going through Slide! Diaphragm Use for Fine Adjustments in High Power Objectives! Fine Adjustment Light Base

Spooky Cells 2 Some are smooth and others are hairy, Frankenstein's was green and scary!

Microscope Lenses Eyepiece Low Power Objective Lens Holds Ocular Lens Magnification of 10X Scanning Objective Lens Magnification of 4x Used for Scanning Use Coarse Focuse Knob Low Power Objective Lens Magnification of 10X Used for Scanning Use Coarse Focus Knob High Power Objective Lens Magnification of 40X Used for Fine Focusing Seeing the details Use Fine Focus Knob ONLY Do NOT use Coarse Focus Knob

The Light Source sends light through the slide. The Iris Diaphragm regulates the amount of light from the light source that passes through the slide. The coarse adjustment should only be used in scanning and low power objectives. It is used for seeing the slide. You should use the fine adjustment while in the high power objective. It is used for detailed focus.

The stage clips hold the slide and are located on the stage which is moved to view different areas of the slide and specimen. All of the objectives are held on the nosepiece which rotates to change the power of magnification. The scanning lens is usually 4x magnification and is used for initial scanning and specimen location on the slide.

The low or medium power objective lens is usually 10x magnification and is also used for scanning. The high power lens is usually 40x or 100x and is used for detailed viewing of a specimen.

Spooky Cell 3 I can make some people scream and squeal, But to Edward Cullen I am a favorite meal.

Fill Your Calculations AND Your answer on Your Worksheet!!! What’s my power? To calculate the power of magnification, MULTIPLY the POWER of the Ocular Lens by the POWER of the Objective What is the power of magnification for the Ocular Lens (shown) and the 40x Objective Lens (shown)? Fill Your Calculations AND Your answer on Your Worksheet!!!

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Do’s and Don’ts of Microscope Labs! USE TWO HANDS Always carry microscopes with one hand on the ARM and the other UNDER the BASE!

Do’s and Don’ts NEVER use Coarse Focus Knob with High Power Lens…it Breaks Slides! “Ooops, I broke a slide” Accidents Happen. Don’t FREAK OUT! Don’t try to clean it up by yourself! Raise your hand and a teacher will help you!

Cleaning UP! Always do 5 Things! Remove Your Slide Wrap Your Cord Lower Your Stage Replace Plastic Cover Put Microscope Back in Designated Area Arm Out

Spooky Cell 4 Stretch me and work me to build more bulk. Soon you will look like the incredible hulk!

SOCRATIVE If you have your own device, go to www.socrative.com click on student log-in and enter room number: 36192

Exit Ticket How well did you understand today’s material? What did you learn today? Problem: What focus power should you always start with?

Wrap Up Activity! Wrap Up Quiz Directions: Log on to a computer Take out a Blank Sheet of Paper Write your name, date, and class in the top write hand corner. Label the paper 1-14 without skipping lines Flip the paper over and label 1-10 on the back 3. Go to: Go to Class Blog and click on link. Bottom Right hand Corner: Microscope Parts Quiz 4. Take the Microscope Parts Quiz Number One! As you answer the questions. Write your answers on your piece of paper. When you have completed the quiz, write your given score at the top of your paper. 5. When you have completed the quiz, go back, and take Microscope Parts Quiz Number Two Fill in your letter answers on the BACK of your sheet of paper. Write your score above your answers!

You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.