Scheme 1 Chemical structures of DMT- BM (LEV) BCE and DMT- CM2 (Bz <sub>2</sub> ) BCE. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides.

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Scheme 1 Chemical structures of DMT- BM (LEV) BCE and DMT- CM2 (Bz <sub>2</sub> ) BCE. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 5 Characterization of 15×3 bAM by HPCE (with co-injection of a purified bAM containing five 60mer sequences). From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 1 ( A ) Standard 15× bDNA comb oligomer Figure 1 ( A ) Standard 15× bDNA comb oligomer. ( B ) 15× bDNA comb oligomer containing CM2 . From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 4 Branched amplification multimer (bAM) assembled by enzymatic ligation. The ligation sites are indicated by ^. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 6 Characterization of 15×3 bAM by BODIPY FL hybridization-dependent quenching. ( A ) Fluorescence of free oligomer-3′-BODIPY FL. ( B ) Fluorescence of oligomer-3′-BODIPY FL hybridized to 15×3 bAM. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 7 Melting curves of AP probe versus complements: ( 1 ) AP probe/1 site complement; ( 2 ) AP probe/3 site complement; ( 3 ) AP probe/15×3 bAM. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 2 Characterization of 15× bDNA comb oligomer Figure 2 Characterization of 15× bDNA comb oligomer. ( A ) HPCE after linear synthesis. ( B ) HPCE after branched synthesis. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press

Figure 3 Characterization of 15× bDNA comb oligomer containing CM2 Figure 3 Characterization of 15× bDNA comb oligomer containing CM2 . HPCE after periodate cleavage. From: Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays Nucleic Acids Res. 1997;25(23):4842-4849. doi:10.1093/nar/25.23.4842 Nucleic Acids Res | © 1997 Oxford University Press