DNA & Protein Synthesis SOL: BIO 6 f - i

SOL: BIO 6 f - i The student will investigate and understand common mechanisms of inheritance and protein synthesis. Key concepts include: f) the structure, function, and replication of nucleic acids (DNA and RNA); and g) events involved in the construction of proteins.

SOL: BIO 6 f - i The student will investigate and understand common mechanisms of inheritance and protein synthesis. Key concepts include: h) use, limitations, and misuse of genetic information; and i) exploration of the impact of DNA technologies.

History Before the 1940’s scientists didn’t know what material caused inheritance. They suspected it was either DNA or proteins.

History A series of experiments proved that DNA was the genetic material responsible for inheritance.

History In 1952, Alfred Hershey and Martha Chase did an experiment using a virus that infects E. coli bacteria. The experiment proved that DNA and not protein is the factor that influences inheritance.

History Erwin Chargaff discovered the base pairing rules and ratios for different species. Adenine pairs with Thymine Cytosine pairs with Guanine.

History Rosalind Franklin & Maurice Wilkins had taken the 1st pictures of DNA using X-ray crystallization

This proved that DNA had a helical shape.

The Nobel Prize in Medicine 1962 History The Nobel Prize in Medicine 1962 Francis Harry Compton Crick James Dewey Watson Rosalind Franklin (Died of cancer 1958) Maurice Hugh Frederick Wilkins

Double Helix Watson Crick Wilkins has become a historical footnote and Watson & Crick are remembered as the Fathers of DNA

DNA O O=P-O N CH2 O C1 C4 C3 C2 Phosphate Group Nitrogenous base (A, T, G, C) CH2 O C1 C4 C3 C2 5 Sugar (deoxyribose)

Nitrogen Bases PGA CUT PY 2 types of Nitrogen Bases Purines Double ring G & A Pyrimidines Single ring C & U & T PGA CUT PY

DNA - double helix T A P O 1 2 3 4 5 P O 1 2 3 4 5 G C T A

DNA The genetic code is a sequence of DNA nucleotides in the nucleus of cells.

DNA DNA is a double-stranded molecule. The strands are connected by complementary nucleotide pairs (A-T & C-G) like rungs on a ladder. The ladder twists to form a double helix.

DNA During S stage in interphase, DNA replicates itself. DNA replication is a semi-conservative process.

DNA Semi-conservative means that you conserve part of the original structure in the new one. You end up with 2 identical strands of DNA.

Good Morning! Please complete the half sheet on the front desk. Please write this in your agenda: TEST on 3/22 (Wednesday). What do I need to study? DNA Structure DNA Replication Protein Synthesis (Transcription/Translation) Mutations (today) DNA Technologies (next class) Today: Mutation Notes Practice and videos Prepare for test on the 22nd!

DNA Gene - a segment of DNA that codes for a protein, which in turn codes for a trait (skin tone, eye color, etc.)

DNA A mistake in DNA replication is called a mutation. Many enzymes are involved in finding and repairing mistakes.

Mutations What causes mutations? Can occur spontaneously Can be caused by a mutagen Mutagen: An agent, such as a chemical, ultraviolet light, or a radioactive element, that can induce or increase the frequency of mutation in an organism.

Mutations Some mutations can: Have little to no effect Be beneficial (produce organisms that are better suited to their environments) Be deleterious (harmful)

Mutations Types of mutations Point Mutations or Substitutions: causes the replacement of a single base nucleotide with another nucleotide Missense- code for a different amino acid Nonsense- code for a stop, which can shorten the protein Silent- code for the same amino acid (AA)

Mutations Example: Sickle Cell Anemia

Mutations Frame Shift Mutations: the number of nucleotides inserted or deleted is not a multiple of three, so that every codon beyond the point of insertion or deletion is read incorrectly during translation Ex.: Crohn’s disease

Deletion

Mutations Chromosomal Inversions: an entire section of DNA is reversed. Ex.: hemophilia, a bleeding disorder

DNA Repair A complex system of enzymes, active in the G2 stage of interphase, serves as a back up to repair damaged DNA before it is dispersed into new cells during mitosis.

Good Afternoon! Warm Up: Copy these facts. **Keep up with all of them for the test. Protein makes up about 20% of the human body; second only to water. The human body contains more than 100,000 different proteins. The structure of certain proteins can trigger allergic reactions in the body. This is why some people are allergic to gluten, a protein found mainly in breads and cereal. Today: Protein Synthesis Notes and videos Class work and Practice

RNA O O=P-O N CH2 O C1 C4 C3 C2 Phosphate Group Nitrogenous base (A, U , G, C ) CH2 O C1 C4 C3 C2 5 Sugar (ribose)

RNA Function: obtain information from DNA & synthesizes proteins

3 differences from DNA Single strand instead of double strand Ribose instead of deoxyribose Uracil instead of thymine

3 types of RNA Messenger RNA (mRNA)- copies information from DNA for protein synthesis Codon- 3 base pairs that code for a single amino acid. codon

3 types of RNA 2. Transfer RNA (tRNA)- collects amino acids for protein synthesis Anticodon-a sequence of 3 bases that are complementary base pairs to a codon in the mRNA

3 types of RNA 3. Ribosomal RNA (rRNA)- combines with proteins to form ribosomes

Amino Acids Amino acids- the building blocks of protein At least one kind of tRNA is present for each of the 20 amino acids used in protein synthesis.

Transcription - mRNA is made from DNA & goes to the ribosome Translation - Proteins are made from the message on the mRNA           

Transcription In order for cells to make proteins, the DNA code must be transcribed (copied) to mRNA. The mRNA carries the code from the nucleus to the ribosomes.

Translation At the ribosome, amino acids (AA) are linked together to form specific proteins. The amino acid sequence is directed by the mRNA molecule. Amino acids ribosome

Make A Protein DNA sequence ATG AAA AAC AAG GTA TAG mRNA sequence UAC UUU UUG UUC CAU AUC

Make mRNA mRNA sequence UAC UUU UUG UUC CAU AUC tRNA sequence AUG AAA AAC AAG GUA UAG

Make mRNA mRNA sequence UAC UUU UUG UUC CAU AUC tRNA sequence AUG AAA AAC AAG GUA UAG Amino Acid sequence Tyr Phe Leu Phe His Ile

Possible Proteins for #4 Amino Acid Sequence Protein Name Function Meth-Glut-Ser-Ala-Isoleu Collagen Elasticity of the skin Meth-Glut-Asp-Leu-Threo-Arg-Ala-Leu Tubulin Makes up the microtubules (cytoskeleton) of the cell. Meth-Asp-Pro-Stop-Leu-Tyr-Ala-Phenyl Actin Protein found in muscle along with myosin. Meth-Asp-Asp.Acid-Ser-Pro-Asp Hemoglobin Carries oxygen on the red blood cells Meth-Leu-Pro-Glut-Leu-Tyr-Ala-Threo-Meth-Tyr Fibronectin Extracellular matrix that holds cells together to make tissues. Meth-Asp-Pro-Ser-Leu-Lys Cyclase Controls the cell cycle and timing of Interphase and Mitosis

Human Genome Project The Human Genome Project is a collaborative effort of scientists around the world to map the entire gene sequence of organisms. This information will be useful in detection, prevention, and treatment of many genetic diseases.

Example of a DNA Sequence: What could you do with this? Transcribe, translate  study the composition of the proteins coded for in this sequence Compare to other human sequences at this position on this chromosome to look for mutations. Compare to the DNA of other organisms.

DNA Technologies DNA technologies allow scientists to identify, study, and modify genes. Forensic identification is an example of the application of DNA technology.

Gene Therapy Gene therapy is a technique for correcting defective genes responsible for disease development. Possible cures for: diabetes cardiovascular disease cystic fibrosis Alzheimer's Parkinson’s and many other diseases is possible.

Genetic Engineering The human manipulation of the genetic material of a cell. Recombinant DNA- Genetically engineered DNA prepared by splicing genes from one species into the cells of a different species. Such DNA becomes part of the host's genetic makeup and is replicated.

Genetic Engineering Genetic engineering techniques are used in a variety of industries, in agriculture, in basic research, and in medicine. This genetically engineered cow resists infections of the udders and can help to increase dairy production.

Genetic Engineering There is great potential for the development of useful products through genetic engineering EX., human growth hormone, insulin, and pest- and disease-resistant fruits and vegetables Seedless watermelons are genetically engineered

Genetic Engineering We can now grow new body parts and soon donating blood will be a thing of the past, but will we go too far? Photo of a mouse growing a "human ear"

Good Afternoon! Welcome Back!  Warm Up- DNA Review Sheet Looking Ahead: This week: finish DNA/RNA/Protein Synthesis Next 3 weeks: Evolution Kingdoms of Life Ecology SOL Review (think Advanced Score!)