Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City,

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Presentation transcript:

Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City, Vietnam Nguyen thi Tham, Vu thi Ty Hang, Trong Huu Khanh, Do Chau Viet, Tran Tinh Hien, Jeremy Farrar, Nguyen van Vinh Chau, H. Rogier van Doorn Diagnostic Microbiology and Infectious Disease Volume 74, Issue 2, Pages 131-136 (October 2012) DOI: 10.1016/j.diagmicrobio.2012.06.003 Copyright © 2012 Elsevier Inc. Terms and Conditions

Fig. 1 Relatedness of results of CDC rRT-PCR and Roche rRT-PCR A/M2 and A/H1N1 assays in nose (A) and throat swabs (B). The x-axis represents the Ct value in CDC rRT-PCR for each specimen. Closed circles represent specimens with a positive Roche rRT-PCR A/H1N1 assay result; closed triangles, specimens with a positive Roche rRT-PCR A/M2 assay result. Open circles and triangles indicate specimens with a negative result for the respective assays. The vertical line is the mean Ct value in CDC rRT-PCR in each group. Diagnostic Microbiology and Infectious Disease 2012 74, 131-136DOI: (10.1016/j.diagmicrobio.2012.06.003) Copyright © 2012 Elsevier Inc. Terms and Conditions

Fig. 2 Correlation of Ct value in CDC rRT-PCR and log10 viral genome copies per milliliter of viral transport medium in Roche rRT-PCR A/M2 assays in positive nose (A) and throat (B) swabs and in Roche rRT-PCR A/H1 assays in nose (C) and throat (D) swabs. Diagnostic Microbiology and Infectious Disease 2012 74, 131-136DOI: (10.1016/j.diagmicrobio.2012.06.003) Copyright © 2012 Elsevier Inc. Terms and Conditions