Article category: Notes Field under which the paper is to be reviewed: Molecular biology   Identification of a novel Arabidopsis mutant showing sensitivity to histone deacetylase inhibitors Nguyen Hoai Nguyen1 • Chan Young Jeong1, 2 • Won Je Lee1, 2 • Hojoung Lee1, 2, * 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea. 2Institute of Life Science and Natural Resources, Korea University, Seoul 136-713, Republic of Korea. *Corresponding author: Hojoung Lee Address: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, Republic of Korea. Telephone: +82-2-3290-3006 Fax: +82-2-3290-3508 Email: lhojoung@korea.ac.kr

Supplementary materials

Figure S1 Col-0 shdi1 Col-0 shdi1 Control Control NaB 200 µM + IAA 5 µM TSA 5 µM + IAA 5 µM Figure S1. Growth of the shdi1 mutant on screening media. Four-day-old seedlings of shdi1 and Col-0 were transferred to the indicated media for 2 weeks.

Recombination frequency (%) Figure S2 (A) No. of F2 seedlings Ratio WT shdi1 shdi1 was crossed with Col-0 163 51 3 1 with Ler 164 53 Table 1. Segregation of nia6 phenotype in F2 (B) SSLP markers on Chr. 5 Recombination frequency (%) nga225 50 AthEMC 36.6 nga249 nga151 40 nga106 41.6 nga76 Figure S2. The shdi1 is a recessive mutant. (A) The shdi1 mutant was crossed with wild-type plants (Col-0 and Landsberg erecta), and the F2 progeny were sown on screening media to test the recessiveness of shdi1 phenotype. (B) For mapping, shdi1 was crossed with the Landsberg erecta ecotype. The location of the shdi1 locus was mapped on the basis of shdi1 phenotypes with several SSLP markers.

Figure S3 (A) (B) Light Dark Col-0 shdi1 * Col-0 shdi1 Hypocotyl length (mm) (A) Col-0 shdi1 Dark (B) Figure S3. The shdi1 mutant showed a shorter hypocotyl than wild-type plants. (A) Seeds of shdi1 and Col-0 were put on 1/2× MS medium and grown in light conditions for 2 weeks. (B) Seeds of shdi1 and Col-0 were put on 1/2× MS medium and grown in dark conditions for 4 days. The asterisks above the columns indicate significant differences in hypocotyl length between shdi1 and Col-0 (P < 0.05).

Table S1. Primers used For RT-PCR Name Forward Primer (5’-3’) Reverse Primer (5’-3’) ACTIN8 ATGAAGATTAAGGTCGTGGCA TCCGAGTTTGAAGAGGCTAC AS1 AGACAGTTCGGTCCGAGAGA GGGAACCTCAGCAGCAATCT AS2 CCTCAACGAGCTTCACCCTT GATAGTGGTCTCTCACGGCG KNAT1 TGACAACAGCACCACTCCTC TTGCTTCCTGAATGGGCCTT PIF4 ATCGGGGATTAGGGAAACAG ATCCTGATCGTTGGTTCGAC IAA17 AAGATCCAGCCAAACCTCCG TGGCAGGAAACCATCACGTT MAX2 TCGACGGTGTGGCTTTATGT AAGCGAGAGACTCCTCTGGT BRC1 GTCTCATATCCGACCGGTGT TGGCTGCGATGGTGGTTAAT DWF4 TGCCGGACATGAGACTTCTT GTTGTTTCCCCACGTCGAAA UBC21 TTACGAAGGCGGTGTTTTTC TGAACCCTCTCACATCACCA