Center of Excellence in Cancer Research (CECR) Director, Dr. Mohamed L. Salem, PhD Prof. of Immunology Tanta University, Egypt

Tanta University Educational Hospital Location Location Tanta University Educational Hospital

Established Date Established Date June 2013 through a project funded by Science and Technology development Fund (STDF) PI: Prof. Mohamed Labib Salem Affiliation: Tanta University

Research Team Dr. Mohamed L.Salem Dr. Mohamed Ramadan Dr. Said Hammad Prof. of immunology Dr. Mohamed Ramadan Prof. of Pediatric Dr. Said Hammad Prof. of clinical Pathology Dr. Mohamed Attia Assis. Prof. of Clinical Pathology Dr. Abdel-Aziz Zidan PhD of Immunology Ms. Mona Fouad Zidan Researcher Assistant

Research Team

Organogram

CECR Main Project Correlating immunologic and clinical performance between genomic, transcriptomic and proteomic profiling of circulating tumor cells versus primary tumor cells

CECR Core Facility

Flow Cytometry Unit BD FACSCanto II Cytometer Nomination: The BD FACSCanto derives its name from the musical term “bel canto” meaning operatic singing that focuses on ease and purity of tone with an agile and precise vocal technique.

Specifications of the instrument The BD FACSCanto system has a capacity for three lasers, eight-parameter detection while capturing particle sizes from 0.5 to 50 μm in diameter. Digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting Multiparameter detection helps in: Advanced cell subset analysis. Multiparameter functional studies. Soluble protein detection. Quantification studies for cell-surface antigens and soluble proteins. Decreases the total sample volume necessary for a specific project as more parameters.

Research & Clinical applications of the instrument Specifications of the instrument Research & Clinical applications of the instrument Immunophenotyping by flow cytometry is an important tool in the diagnosis and staging of patients with a haematological neoplasm as well as solid tumors. Some of the flow cytometry applications in diagnosis of different diseases are listed below: Analysis of Leukemias and Lymphomas Detection of minimal residual disease Stem cell enumeration Analysis of different types of solid tumors. Detection of autoantibodies BD FACSCanto Applications Reticulocyte analysis Detection of Immunodeficiency diseases Detection of HIV infection

Cell Sorting Unit The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high- quality , viable cells in a high density format , ready for use in the application of your choice Benefits: Access a wide range of samples. Use your own capture criteria. Capture intact, pure cells. Minimize Sample Diluation Save Time Save Space Utilize validated CTC analysis workflows.

Cell Sorting Unit IsoFlux Applications Enumeration Immunofluorescence Fluorescence in situ hybridization Next generation sequencing Mutation detection Gene expression

Genomic & Proteomic Unit Microarray A high throughput technology that allows detection of thousands of genes simultaneously. Features: High samples throughput. Laser excitation at 532nm, dual- laser excitation . Scanning pixel resolution 2µm Scan sequence: simultaneous scan and independent scan. Scan/read time: approximately 5 samples/hour.

Microarry Applications Genomic & Proteomic Unit Microarry Applications Transiptome mRNA Expression miRNA Expression Splice Variance Genome Structure DNA Copy number Genome Variation Single Nucleotide Polymorphism Copy number Variations Proteome Protein-Protein Interactions DNA – Protein inreraction Epigenetics DNA Methylation Chromatin Structure

Genomic & Proteomic Unit LumineX200

Genomic & Proteomic Unit Flexible Multiplexing Technology Open Platform for Genomic & Proteomic assays Life Science Research & Diagnostic assays available. Broad menu of commercial assays and laboratory developed tests Multiplex up to 500 tests in one well of microliter plate. Decrease sample volume requirements. Reduce assay reagent volume, expense & labor. Generate more information on interrelationships between analytes within a single sample.

Assay Development Tools Genomic & Proteomic Unit Luminex Applications Clinical Diagnostics Clinical Research Life Science Research Biosurveillance Assay Development Tools

CECR Collaborators CECR Collaborators

CECR Conferences Monday 24 February 2014 The 1st Annual Conference of The Center of Excellence in Cancer Research Next Generation Cancer Research & Diagnosis In Collaboration with Research Centers of Excellence (STDF- Affiliated Centers)