exRNA Metadata Standards Donor Sex, Age, Donor Type Family Submission Submitter Name, Email, PI Name, PI Email, Organization, Funding source, Grant details Pools Sample Biological Sample Elements: Species, Disease Type, Anatomical Location, Biofluid, Collection Method, Tube Type, Type of Cell culture Supernatant, Cell Source, Tissue source, Tissue Type Molecular Sample Elements: exRNA Source, Fractionated? Sample Name, Donor ID, Related Experiment qPCR Targets Biosample ID, Related Run, Target Name, Ct Value Study Title, Type, Abstract, Authors, Related Submission Analysis Type (small RNA-seq, qPCR), Genome Version, Number of mapped reads, Alignment software, version, Abundance measurement software, version, qPCR Analysis, Related Biosamples Jobs FTP Job ID, Submission ID, Study ID, Biosample IDs, Analysis ID, Experiment ID, Run ID Experiment exRNA Source Isolation Protocol Description, Cell Type marker, Serum used?, Serum type, Cell removal step done for cell culture supernatant and biofluid? exRNA Sample Prep Protocol Description, Pre-purification of EV done?, RNA Isolation Kit, exRNA Quantification Method Experiment Type (mRNA, smRNA-seq) Library Generation Starting material Type, Library Construction Kit, Strand specificity, Reverse Transcription Kit, Primer Type, Amplified?, DNA Quantification method, smRNA Size selection method Extracellular Vesicle Isolation Protocol: Description, Kits used for EV isolation qPCR: Reverse Transcription, Oligos, Kits, Complete Reaction Conditions, Reaction Volume, Validation Run Experimental Design, Type (small RNA-seq, qPCR), Raw Data Files, Related Study, Related Biosamples 6 letter code for PI, Submitter First letter of First Name, First 4 letters of Last Name + Number (from 1) Example: KJENS1, KJENS2 & so on Doc IDs: EXR-KJENS100000001-SU EXR-KJENS100000001-BS EXR-KJENS100000002-BS EXR-KJENS100000001-EX PI Codes PI Names, Submitter List, PI Code

Metadata Submission Guidelines Submit 1 Submission document – UNIQUE for each Submitter-PI-Grant combination – REUSE for submissions from same user/PI/grant - Do NOT provide duplicates. Submission Submit 1 Study document with a unique title and overall description for a single research initiative – This describes “why” you sequenced your samples (similar to NCBI BioProject) – REUSE for all samples sequenced under a single study. Study Submit 1 Run document providing a list of data files that are part of the submission – UNIQUE for each FTP submission. Run Submit 1 or more Experiment document(s). Ensure Biosample is linked with the correct Experiment document ID. REUSE for experiments that followed exact same protocols. Experiment Submit one or more Donor document(s) for all Biosamples that are part of this submission – UNIQUE for each Donor – REUSE for samples from same donor - Do NOT provide duplicates. Donor Submit 1 Biosample document for each data file submitted. Ensure the correct Donor ID is provided in each Biosample document. Biosample Optionally submit 1 Analysis document – If not provided with submission, it will be generated and relevant fields along with Biosamples will be populated by the FTP submission pipeline – UNIQUE for each FTP submission. Analysis Submit 1 qPCR Target for each Biosample. Should contain Target Names and the corresponding Ct values. qPCR Targets (only for qPCR Assays)