FIG. 1. Effect of BDCM on the organization of desmosomal proteins in trophoblast cultures. Cytotrophoblast cells were incubated with or without BDCM under.

Slides:



Advertisements
Similar presentations
Nuclei number/field Percent multinucleation MediumBr-cAMP Percent TUNEL positive Fresh cells Frozen.
Advertisements

High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,
Chrysin restores PDGF-induced inhibition on protein tyrosine phosphatase and reduces PDGF signaling in cultured VSMCs  Huey-Ming Lo, Min-Wen Wu, Shiow-Lin.
CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation  G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.
From: Elevated Expression of O-GlcNAc–Modified Proteins and O-GlcNAc Transferase in Corneas of Diabetic Goto-Kakizaki Rats Invest. Ophthalmol. Vis. Sci..
Neuroprotective Effect of Didymin on Hydrogen Peroxide-Induced Injury in the Neuronal Membrane System Cells Tissues Organs 2014;199: DOI: /
Volume 36, Issue 8, Pages (August 2015)
Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites  Bo Peng, Ph.D.,
L. Nikitina, F. Wenger, M. Baumann, D. Surbek, M. Körner, C. Albrecht 
TIA-1 Self-Multimerization, Phase Separation, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+  Joseph B. Rayman, Kevin A. Karl,
High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,
Moderate Hypoxia Down-Regulates Interleukin-6 Secretion and TLR4 Expression in Human Sw.71 Placental Cells Cell Physiol Biochem 2015;36: DOI: /
Triterpenes Promote Keratinocyte Differentiation In Vitro, Ex Vivo and In Vivo: A Role for the Transient Receptor Potential Canonical (subtype) 6  Ute.
Volume 20, Issue 5, Pages (May 2011)
CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation  G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.
AβOs bind to and impact hypothalamic neurons Representative immunocytochemistry images of mature hypothalamic neurons in culture exposed to vehicle (Veh)
Hyaluronate-Enhanced Hematopoiesis: Two Different Receptors Trigger the Release of Interleukin-1β and Interleukin-6 From Bone Marrow Macrophages by Sophia.
Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells  Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,
Defective killing of Staphylococcus aureus in atopic dermatitis is associated with reduced mobilization of human β-defensin-3  Kevin O. Kisich, PhD, Charles.
Volume 69, Issue 8, Pages (April 2006)
Volume 96, Issue 10, Pages (May 2009)
John F. Öhd, Katarina Wikström, Anita Sjölander  Gastroenterology 
I LC3 II a-Tubulin Figure S1 Ctrl Myoblasts 0h shMLP Myoblasts 0 h
Volume 63, Issue 3, Pages (March 2003)
Plakoglobin Deficiency Protects Keratinocytes from Apoptosis
Molecular Therapy - Methods & Clinical Development
Volume 124, Issue 5, Pages (May 2003)
Volume 120, Issue 7, Pages (June 2001)
Assessment of strategies to increase chondrocyte viability in cryopreserved human osteochondral allografts: evaluation of the glycosylated hydroquinone,
Inhibition of lysyl oxidase activity can delay phenotypic modulation of chondrocytes in two-dimensional culture  J. Farjanel, Ph.D., S. Sève, Ph.D., A.
Very low-density lipoprotein stimulates the expression of monocyte chemoattractant protein-1 in mesangial cells  Edward G. Lynn, Yaw L. Siow, Dr Karmin.
Volume 27, Issue 1, Pages (July 2007)
Glutamine protects articular chondrocytes from heat stress and NO-induced apoptosis with HSP70 expression  H. Tonomura, M.D., K.A. Takahashi, M.D., Ph.D.,
Leukemia inhibitory factor increases the invasiveness of trophoblastic cells through integrated increase in the expression of adhesion molecules and pappalysin.
S.-M. Harwood, D.-A. Allen, M.-J. Raftery, M.M. Yaqoob 
by Silvia Mele, Stephen Devereux, Andrea G
Nachiket Shembekar, Hongxing Hu, David Eustace, Christoph A. Merten 
Alex M. Agelidis, Satvik R. Hadigal, Dinesh Jaishankar, Deepak Shukla 
Induction of Adipose Differentiation Related Protein and Neutral Lipid Droplet Accumulation in Keratinocytes by Skin Irritants  Emmanuela Corsini, PhD,
Microtubule-Targeted Drugs Inhibit VEGF Receptor-2 Expression by both Transcriptional and Post-Transcriptional Mechanisms  Markus Meissner, Andreas Pinter,
Fig. 1 BX795 suppresses HSV-1 infection.
Identification of Small Molecule Inhibitors that Distinguish between Non-Transferrin Bound Iron Uptake and Transferrin-Mediated Iron Transport  Jing Xu.
Volume 103, Issue 11, Pages (December 2012)
Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin  Y. Luan, Ph.D., M.D., L. Kong, Ph.D.,
Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease  Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,
Serotonin Regulates the Secretion and Autocrine Action of a Neuropeptide to Activate MAPK Required for Long-Term Facilitation in Aplysia  Jiang-Yuan Hu,
Raymond L. Warters, Patrick J. Adamson, Christopher D. Pond, Sancy A
Cholera Toxin FhHDM-1 Merged
Volume 61, Issue 5, Pages (May 2002)
Volume 8, Issue 4, Pages (April 2017)
4-Tertiary Butyl Phenol Exposure Sensitizes Human Melanocytes to Dendritic Cell- Mediated Killing: Relevance to Vitiligo  Tara M. Kroll, Hemamalini Bommiasamy,
Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature  Jessica S.
Increased Expression of Wnt2 and SFRP4 in Tsk Mouse Skin: Role of Wnt Signaling in Altered Dermal Fibrillin Deposition and Systemic Sclerosis  Julie Bayle,
Volume 76, Issue 1, Pages (July 2009)
Martin A. Turman, Scott L. Rosenfeld  Kidney International 
TIA-1 Self-Multimerization, Phase Separation, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+  Joseph B. Rayman, Kevin A. Karl,
Decreased Phospholipase D (PLD) Activity in Ceramide-Induced Apoptosis of Human Keratinocyte Cell Line HaCaT  Yoshihiko Iwasaki-Bessho, Yoshiko Banno,
Cytotoxic T Lymphocyte Antigen-4 Accumulation in the Immunological Synapse Is Regulated by TCR Signal Strength  Jackson G. Egen, James P. Allison  Immunity 
Cell-Shape Regulation of Smooth Muscle Cell Proliferation
Sequential effects of high glucose on mesangial cell transforming growth factor-β1 and fibronectin synthesis  Jong Hoon Oh, Hunjoo Ha, Mi Ra Yu, Hi Bahl.
Interleukin 6 Indirectly Induces Keratinocyte Migration
MPP+-induced toxicity in cultured SN and VTA DA neurons.
Unmodified Cadmium Telluride Quantum Dots Induce Reactive Oxygen Species Formation Leading to Multiple Organelle Damage and Cell Death  Jasmina Lovrić,
CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4  Kandace Bonner,
Association of NM-HA and NM-GFP with SGs is transient.
Volume 103, Issue 11, Pages (December 2012)
Yann Leverrier, Anne J. Ridley  Current Biology 
Volume 94, Issue 1, Pages (January 2008)
CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4  Kandace Bonner,
A, Pharmacologically inhibiting protein tyrosine kinases significantly reduces the viability of ATRT cell lines as compared with control HEK 293 cells.
Presentation transcript:

FIG. 1. Effect of BDCM on the organization of desmosomal proteins in trophoblast cultures. Cytotrophoblast cells were incubated with or without BDCM under differentiation-inducing conditions (i.e., in KGM) for 48 h and then stained to reveal nuclei (red fluorescence) and desmosomal proteins (green fluorescence) as described in Methods. (A) Control nondifferentiated cytotrophoblast colonies. (B) Control differentiated syncytiotrophoblast-like colonies. The images in the other panels show trophoblasts cultured under differentiation-inducing conditions in the presence of BDCM. The concentrations were 2.0 mM (C); 0.2 mM (D); 0.02 mM (E) and 0.5 nM (F). The images are representative of samples from three separate experiments (cells from three different placentas). The horizontal bar represents 50 μm. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology

FIG. 2. Effect of BDCM on the formation of multinucleated trophoblast colonies. Cytotrophoblast cells were incubated with or without BDCM under differentiation-inducing conditions for 48 h. The data indicated by 0(K) and 0(H) represent control cultures incubated in KGM or HWM, respectively, in the absence of BDCM. The cultures were then stained to reveal nuclei and desmosomal proteins as described in Methods and Figure 1. Fluorescence images were captured, and the total numbers of nuclei were counted in random fields. At least 150 nuclei were counted in each case. The extent of multinucleated cell (i.e., syncytiotrophoblast) formation was calculated as described in Methods. Results are means ± SEM. from three separate experiments. Asterisks indicate values significantly different (p < 0.05) from untreated controls. Post-ANOVA trend analysis indicates a significant linear trend. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology

FIG. 3. Effect of BDCM on the secretion of immunoreactive and bioactive CG. Cytotrophoblasts were cultured for 48 h under differentiation-inducing conditions (i.e., in KGM) in the presence or absence of BDCM. The culture media were collected and analyzed for immunoreactive (A) and bioactive (B) CG as described in the Materials and Methods section. Results are means ± SEM from four separate experiments (four different placentas). The asterisks indicate values that are significantly different (p < 0.05) from the control. Post-ANOVA trend analysis indicates a significant linear trend for both datasets. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology

FIG. 4. Immunofluorescence localization of CG in trophoblasts exposed to BDCM. Cytotrophoblasts were cultured for 48 h under differentiation-inducing (KGM) conditions in the presence or absence of BDCM. The cultures were then fixed and stained using an anti-CG antibody as described in the Methods section. (A) Control cells incubated under non-differentiation-inducing conditions (B) Control cells incubated under differentiation-inducing conditions. The other images show cells incubated under differentiation-inducing conditions in the presence of different concentrations of BDCM. The concentrations used were 2.0 mM (C), 0.2 mM (D), 0.02 mM (E), 200 nM (F), and 0.5 nM (G). The last image (H) shows the immunoglobulin control. The horizontal bar represents 50 μm. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology

FIG. 5. Image analysis of CG immunofluorescence FIG. 5. Image analysis of CG immunofluorescence. Fluorescence micrographs from the experiment described in Figure 4 were imported into Image Pro software as described in Materials and Methods. Mean green fluorescence intensity values were obtained. Results are means ± SEMs from three separate experiments. At least 125 cell colonies were analyzed in each case. The data indicated by 0(K) and 0(H) represent control cultures incubated in KGM or HWM, respectively, in the absence of BDCM. The asterisks indicate values that are significantly different (p < 0.05) from the differentiated (K) control culture. ANOVA post-tests also showed a significant linear trend. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology

FIG. 6. Effect of BDCM on trophoblast viability FIG. 6. Effect of BDCM on trophoblast viability. Trophoblasts were cultured for 48 h under differentiation-inducing conditions in the presence or absence of BDCM. After removal of culture supernatants, the protein content of the adherent cells was measured (A). Culture supernatants were assayed for LDH activity (B) as described in Materials and Methods. Results are means ± SEM from three separate experiments. ANOVA showed no significant difference between means. From: Bromodichloromethane Inhibits Human Placental Trophoblast DifferentiationDisclaimer: The information in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Toxicol Sci. 2004;78(1):166-174. doi:10.1093/toxsci/kfh046 Toxicol Sci | Society of Toxicology