Corresponding author e-mail: nadertanideh@yahoo.com MRI evaluation for repairing effects of adipose-derived stem cells on cartilage defects of rabbit knee.

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Corresponding author e-mail: nadertanideh@yahoo.com MRI evaluation for repairing effects of adipose-derived stem cells on cartilage defects of rabbit knee joints M. Babazadeh1, D. Mehrabani2, N, Tanideh2, S, Ahrari1, A, Tamadon1, S. Hoseinzadeh3, M, Heidari1, O. Kuhi4, F. Parvin2. 1- Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran 2- Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran 3- Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran 4- Center of Laboratory Animal , Shiraz University of Medical Sciences, Shiraz, Iran Corresponding author e-mail: nadertanideh@yahoo.com Objectives Results The rabbit knee joints were underwent dynamic changes during the process of tissue repair in the defective area after operation. No statistically significant differences were detected between BLOKS scoring system characteristics of all the groups (P>0/05). Control groups showed a deal amount of intra-articular effusion with obvious peripheral tissue edema and a majority of bone marrow lesions. However, these characteristics were not present in treatment groups. There are limited treatment options for cartilage defects in clinical practice because of the lack of suitable biomaterials. Adipose stem cells (ASCs) are believed to be multipotent in differentiation into chondrogenic lineage. This study assessed the effectiveness of intra-articular injection of autologous ASCs to repair a full-thickness articular cartilage defect in the medial femoral condyle of rabbits to augment healing of defects. A A Figure 1. Adipose Stem cell cultures of rabbit . A) passage 2, mag. ×10. B Methods Conclusion A B The results showed that injection of ASCs prevent progression of lesions in treatment groups whereas there were no repairing effects of ASCs in control group A full-thickness (without penetration of the subchondral bone, with 4 mm in diameter) cartilage defect was created in the medial femoral condyle of twelve adult Dutch rabbits. Allogeneic ASCs harvested from subcutaneous adipose tissue were injected intra-articularly after the creation of the defect. Defects without treatment were used as controls. The rabbits were sacrificed 4 and 8 weeks after surgery for MRI analysis using BLOKS scoring system. C D C D Figure 2. MRI illustration of medial condyle of femur of rabbit . A) Control group, 4 weeks after surgery. B) Treatment group, 4 weeks after surgery. C) Control group, 8 weeks after surgery. D) Treatment group, 8 weeks after surgery.