Illumina library construction and sequencing process

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Illumina library construction and sequencing process Illumina library construction and sequencing process. Panel A represents the library construction process whereby high-molecular-weight genomic DNA is fragmented, ligated with adaptors, and amplified on a solid support prior to annealing of adaptor-complementary primers. Panel B represents the stepwise sequencing process whereby reagents are introduced to extend the primed fragments, the incorporated fluorescent nucleotides are detected, the 3′ end is deblocked, and the fluorescent groups on the incorporated nucleotides removed prior to the next stepwise sequencing-by-synthesis series. (Reproduced with permission from Mardis, ER: Next-generation sequencing platforms. Annu Rev Anal Chem (Palo Alto Calif) 2013;6:287-303.) Source: Genomics, Williams Hematology, 9e Citation: Kaushansky K, Lichtman MA, Prchal JT, Levi MM, Press OW, Burns LJ, Caligiuri M. Williams Hematology, 9e; 2015 Available at: http://hemonc.mhmedical.com/DownloadImage.aspx?image=/data/books/1581/kau_ch11_f001.png&sec=94302348&BookID=1581&ChapterSecID=94302337&imagename= Accessed: October 29, 2017 Copyright © 2017 McGraw-Hill Education. All rights reserved