Screening of Anti-inflammatory Drugs

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Presentation transcript:

Screening of Anti-inflammatory Drugs Lab # 4

Outline Describe the inflammatory response Anti-inflammatory drugs; MOA, Uses, SE Explain methods for screening of Anti-inflammatory drugs Discuss the lab work 14-03-07 Gamra Hamad

Inflammation Response of immune system to foreign organism or antigenic substance Characterized by edema, erythema, swelling, and pain or tenderness 14-03-07 Gamra Hamad

Inflammatory Mediators Phospholipids http://nnsearch.qsrch.com/shp.php 14-03-07 Gamra Hamad

Phases of inflammation Acute: vasodilation & increase capillary permeability Delayed: infiltration of leukocytes and phagocyte cells Chronic proliferative: tissue degeneration and fibrosis 14-03-07 Gamra Hamad

Anti-inflammatory Drugs Steroidal: ex. Corticosteroids (inh. gene transcription ) Non-steroidal Anti-inflammatory Drugs (NSAID) (inh. COX) Examples: ASA, indomethacin, ibuprofen Selective COX-2 inhibitors 14-03-07 Gamra Hamad

NSAID Uses: rheumatoid arthritis SE: GI adverse effects, renal insufficiency, bronchospasm 14-03-07 Gamra Hamad

Screening methods For Anti-inflammatory Drugs Granuloma Pouch edema methods Croton oil Control vs. treated Calculate % inhibition Adjuvant-induced arthritis ID Tubercle bacilli Calculate % change in edema & body wt. over 14 days 14-03-07 Gamra Hamad

Screening methods For Anti-inflammatory Drugs Rat-paw edema Animal 6 rats (3 control, 3 treated) Drugs: carragenin (2%, 0.1 ml SC.), indomethacin 2 mg/kg IP 14-03-07 Gamra Hamad

Cont. Control group Treated group Rt. Lt. 1 2.9 1.5 1.9 1.3 2 3.1 1.7 1.4 3 1.6 Mean A B C D 14-03-07 Gamra Hamad

Calculation % of edema produced by carragenin alone = A – B X 100 = X % B % of edema produced by carragenin after ttt with indomethacin = C – D X 100 = Y % D % reduction of edema by indomethcin = X –Y= % 14-03-07 Gamra Hamad