Microscopes.

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Presentation transcript:

Microscopes

Care for your microscope Always carry your scope with 2 hands (One around the handle/Arm; One supporting the base) The Handle/Arm and oculars should be facing you when using the microscope. Only use lens paper to clean the objectives. (provided in each Student Drawer) Store the dust cover in your drawer/cabinet so it is out of the way Always use the plastic mat provided under your microscope

Microscope focusing Initial Focusing Use Slide #3: Bacteria and Blood Place slide between stage clips, label facing up Turn on the light until you have a WHITE background. (CONDENSER should be all the way up) View the slide through the OCULARS with both eyes. (adjust the for the distance between your eyes until you are able to see one field of vision) Use the STAGE ADJUSTMENT KNOBS until the specimen is in the middle of the light. Use the COURSE FOCUS to bring the specimen into focus. Start with the slide all the way and turn the coarse adjustment know AWAY from you.

Moving to the next higher magnification (10x) Parfocal: the specimen will be in partial focus when moving to the next higher magnification Move the revolving nosepiece clockwise to the next objective (Low Power-10X) Once specimen is into focus, you can move to the next higher objective (40x) by repeating the above steps Use only the FINE FOCUS for High-Power (40x) and higher magnifications to bring the specimen into focus (Using the course focus could cause damage to the objective)

Care of the Microscope The High-Dry/HighPower objective is to NEVER touch oil.

Oil Immersion Once your specimen is brought to focus under the High-Dry, you can use Immersion oil for the oil objective. Move the revolving nosepiece to the scanning objective (40x→10x→4x) DO NOT lower the stage. The stage and lighting is set for the best view of the specimen already. Add a drop of immersion oil onto the slide where light is passing through Rotate the revolving nosepiece counterclockwise (4x→100x) and adjust the focus using ONLY the FINE FOCUS. You can adjust the diaphragm to allow more light to pass

Storing your Microscope Scanning Objective over the Stage Oil Immersion Objective COMPLETELY wiped clean of oil Center the stage Stage in the full down position Scope switched off Phase Adapter in the OUT position Cord Wrapped around cord holder Dust Cover on Microscopes are stored with the handle facing out