To supply the growth requirements

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Presentation transcript:

To supply the growth requirements Culture Media To supply the growth requirements

overview of cell metabolism: catabolic: energy releasing anabolic: energy requiring macronutrients include: C, N, P, S, K, Na micronutrients : Ca, Mg, Fe Trace elements, B, Zn. Cr, Co, Cu, Mn, V, W Growth factors: vitamins

phototrophs: light as energy source Recall: energy classes of microorganisms phototrophs: light as energy source chemotrophs: chemicals as energy source chemoorganotrophs: organic chemicals chemolithotrophs: inorganic chemicals

Culture Media Defined: made from purified chemicals e.g., glucose-mineral salts broth Complex (undefined): exact chemical composition not known e.g., YE (yeast extract) agar Enriched extra range of nutrients eg Blood agar agar – polysaccharide derived from algae forms a gel at temperatures of interest, including 37ºC not many microorganisms can degrade it widely used as solidifying agent in microbial media

aseptic transfer:

Liquid Solid

streak plate:

Types of Agar Media Enriched media Differential and selective media Contain growth factors and other complex organic substances like blood, serum, etc Better for FASTIDIOUS bacteria Differential and selective media Powerful tools in clinical lab Some can give preliminary ID of bacteria

Differential Media

Selective Media Mannitol Salt Agar MacConkey Agar

Miscellaneous Media Examples of miscellaneous media are reducing, fermentation and transportation media.