A B Bait site: Jk5-coding end (CE)

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A B Bait site: Jk5-coding end (CE) Results based on HTGTS-Rep-seq pipeline A Vks (~160) Jks (5) Cen. Tel. Jk5 Non-productive Productive B 33% Wild-type v-Abl line Vk1-88 Vk4-86 67% 32% E88 v-Abl line mm9 68% 39% Wild-type small pre-B mm9 E88 small pre-B Vk4-86 Vk1-88 61% 36% 64%

A B Bait site: Jk4-coding end (CE) Results based on HTGTS-Rep-seq pipeline A Cen. Tel. Jks (5) Vks (~160) Non-productive Productive Jk4 B 36% Wild-type v-Abl line mm9 E88 v-Abl line Vk4-86 Vk1-88 64% 33% 67% Vk1-88 38% Wild-type small pre-B mm9 6 8 E88 small pre-B Vk4-86 62% 38% 62%

A B Bait site: Jk2-coding end (CE) Results based on HTGTS-Rep-seq pipeline A Cen. Tel. Jks (5) Vks (~160) Non-productive Productive Jk2 B 30% Wild-type v-Abl line mm9 E88 v-Abl line Vk1-88 Vk4-86 70% 33% 67% 41% Wild-type small pre-B mm9 E88 small pre-B Vk1-88 Vk4-86 59% 39% 61%

A B Bait site: Jk1-coding end (CE) Results based on HTGTS-Rep-seq pipeline A Cen. Tel. Jks (5) Vks (~160) Jk1 Non-productive Productive B 41% Wild-type v-Abl line Vk1-88 Vk4-86 59% 33% E88 v-Abl line mm9 67% Wild-type small pre-B Vk1-88 50% Vk4-86 50% 41% E88 small pre-B mm9 59%

Bait site: Jk5-coding end (CE) Results based on HTGTS translocation pipeline and normalized to the same junction numbers. Vks Jks Igk annotation* Vk1-88 Vk4-86 (+) 460 Wild-type v-Abl line# (-) 481 (+) 460 E88 v-Abl line (-) 481 (+) 988 Wild-type small pre-B$ (-) 1358 (+) 988 E88 small pre-B (-) 1358 * Red bars denote Vks with RSSs the same directions as the downstream Jk RSSs; blue bars denote Vks with RSSs convergently to the downstream Jk RSSs. Grey bars denote pseudo/ORF Vks. # (+) means inversional joining between Jk RSSs and the Vks RSSs that are in the same direction as Jk RSSs. (-) means deletional joining between Jk RSSs and the convergent Vks RSSs. Between these two v-Abl lines with Jk5-CE bait primer, the total junction numbers were normalized to 21,398. $ Between these small pre-B lines with Jk5-CE bait primer, the total junction numbers were normalized to 50,549.

Bait site: Jk4-coding end (CE) Results based on HTGTS translocation pipeline and normalized to the same junction numbers. Vks Jks Igk annotation (+) 250 Vk1-88 Vk4-86 Wild-type v-Abl line # (-) 250 (+) 250 E88 v-Abl line (-) 258 (+) 233 Wild-type small pre-B $ (-) 377 (+) 233 E88 small pre-B (-) 377 # Between these two, v-Abl lines with Jk4-CE bait primer, the total junction numbers were normalized to 8,230. $ Between these small pre-B lines with Jk4-CE bait primer, the total junction numbers were normalized to 7,705.

Bait site: Jk2-coding end (CE) Results based on HTGTS translocation pipeline and normalized to the same junction numbers. Vks Jks Igk annotation Vk1-88 Vk4-86 (+) 84 Wild-type v-Abl line # (-) 200 (+) 84 E88 v-Abl line (-) 200 (+) 245 Wild-type small pre-B $ (-) 338 (+) 245 E88 small pre-B (-) 338 # Between these two, v-Abl lines with Jk2-CE bait primer, the total junction numbers were normalized to 6,765. $ Between these small pre-B lines with Jk2-CE bait primer, the total junction numbers were normalized to 9,288.

Bait site: Jk1-coding end (CE) Results based on HTGTS translocation pipeline and normalized to the same junction numbers. Vks Jks Igk annotation Vk1-88 Vk4-86 (+) 115 Wild-type v-Abl line # (-) 206 (+) 115 E88 v-Abl line (-) 206 (+) 153 Wild-type small pre-B $ (-) 152 (+) 153 E88 small pre-B (-) 152 # Between these two, v-Abl lines with Jk1-CE bait primer, the total junction numbers were normalized to 4,449. $ Between these small pre-B lines with Jk1-CE bait primer, the total junction numbers were normalized to 4,167.