From: Quantitative Assessment of UVA-Riboflavin Corneal Cross-Linking Using Nonlinear Optical Microscopy Invest. Ophthalmol. Vis. Sci.. 2011;52(7):4231-4238.

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From: Quantitative Assessment of UVA-Riboflavin Corneal Cross-Linking Using Nonlinear Optical Microscopy Invest. Ophthalmol. Vis. Sci.. 2011;52(7):4231-4238. doi:10.1167/iovs.10-7105 Figure Legend: (a) Indentation force measurement apparatus was composed of an x–y translation stage supporting the cornea holder (expanded view), which clamped the cornea between two metal plates with an O-ring. Corneal stiffness was measured with a force transducer with 250-μm diameter indenting probe attached to a z-axis translation stage. (b) After measuring stiffness, the corneas were fixed, bisected through the central cornea, embedded in agarose, and vibratome sectioned to obtain a 300-μm-thick central corneal slice. (c) The central regions of the vibratome sections were scanned with nonlinear optical microscopy. Date of download: 10/29/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.