No Pain, No Gain: Understanding Muscle Pain

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Presentation transcript:

No Pain, No Gain: Understanding Muscle Pain Jankowski Lab – Anesthesia/Pain Management Jordan Lamb

Project Description Goal – Better understand molecular mechanisms that lead to muscle pain after injury in order to determine better treatments for musculoskeletal pain Muscle-specific injury model in mice Block blood flow in right forepaw by tying off brachial artery (Ischemia) Remove blockage after several hours to allow blood flow again (reperfusion) Causes changes in the muscle tissue and in gene expression in afferent neurons that carry pain input from muscle to CNS

Project Description Cont. Three types of procedures to study pain produced after injury Behavior – guarding, grip strength, threshold they respond to mechanical stimuli Electrophysiological recording – responsiveness to mechanical, thermal, and chemical muscle stimulation Molecular Assays My role Focus on molecular assays Real-time PCR analysis

What? So What? Now What? Basic laboratory skills – pipetting, weighing chemicals, preparing solutions, mouse handling, etc. Exposed to variety of different procedures RNA isolation, protein isolation, reverse transcription, PCR, cell culture Assist with animal surgery About myself Like working in structured environment – following procedure Gaining independence Asking for help

What? So What? Now What? Bettering understanding of neuroscience Classroom and daily life Chance to explore other science/medicine related careers Perceptions on research process A LOT of work More respect Prepared to continue research this summer and in the future