Date of download: 10/30/2017 Copyright © ASME. All rights reserved. From: Ultraviolet-Crosslinkable and Injectable Chitosan/Hydroxyapatite Hybrid Hydrogel for Critical Size Calvarial Defect Repair In Vivo J. Nanotechnol. Eng. Med. 2016;6(4):041001-041001-6. doi:10.1115/1.4032902 Figure Legend: Synthesis, characterization, and property of UV-crosslinkable and injectable N-MAC. (a) The N-acylation scheme between chitosan and MA; (b) 1H NMR spectra (400 MHz, D2O) of N-MAC; (c) FTIR spectra of chitosan, N-MAC, and N-MAC/HA; (d) photos of N-MAC solution in inclined vial (up); N-MAC solution could form hydrogel that exhibited fixed shape, respectively, in inverted vial (down) after UV irradiation; (e) SRs of N-MAC hydrogel with DS of 28.4%; ESEM photograph of N-MAC hydrogel; (f) the XRD patterns of chitosan, N-MAC and N-MAC/HA. (g) Degradation of N-MAC and N-MAC/HA hydrogels in PBS containing 1 mg/mL papain at 37 °C.

Date of download: 10/30/2017 Copyright © ASME. All rights reserved. From: Ultraviolet-Crosslinkable and Injectable Chitosan/Hydroxyapatite Hybrid Hydrogel for Critical Size Calvarial Defect Repair In Vivo J. Nanotechnol. Eng. Med. 2016;6(4):041001-041001-6. doi:10.1115/1.4032902 Figure Legend: The biocompatibility of N-MAC solution and N-MAC hydrogel. (a) NIH/3T3 cells viability of N-MAC solution with different concentrations for culture 12 hrs and culture medium was used as control group. Each point is presented as mean±standard deviation (SD) (n = 5, *p < 0.01); (b) proliferation of NIH/3T3 cells in the presence of N-MAC solution for culture 12, 24, and 36 hrs. Each point is presented as mean±SD (n = 5); (c) fluorescence images of 3T3 cell in N-MAC hydrogel. (d) Typical photos of N-MAC hydrogel adjacent with regional skin after 6 days. H&E stained histological images of subcutaneous tissue at 10 days.

Date of download: 10/30/2017 Copyright © ASME. All rights reserved. From: Ultraviolet-Crosslinkable and Injectable Chitosan/Hydroxyapatite Hybrid Hydrogel for Critical Size Calvarial Defect Repair In Vivo J. Nanotechnol. Eng. Med. 2016;6(4):041001-041001-6. doi:10.1115/1.4032902 Figure Legend: Critical size calvarial defect repair using UV-crosslinkable and injectable hybrid hydrogel. (a) Representative X-ray images of isolated calvaria of only N-MAC hydrogel, N-MAC 20, N-MAC 40, and N-MAC 100 4 weeks (a) and 6 weeks (b); the statistical evaluations of apparent repair area of only N-MAC hydrogel, N-MAC 20, N-MAC 40 and N-MAC 100 after 4 weeks (c) and 6 weeks (d).