Plant Tissue Culture.

Slides:

Advertisements

Similar presentations

Agricultural Biotechnology

Advertisements

Preparing Plant Tissue Culture Medium

Biotechniques. Tissue Culture (micro-propagation) A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole.

Organogenesis in Peanut Research Experience for Undergraduates Food Biotechnology Laboratory Alabama A&M University Antonio Brazelton 7/3/08.

Plant Tissue Culture Do you want a footer?.

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Plant Cell, Tissue and Organ Culture Cell Suspension Cultures

Wed Lab 5 discussion: Discuss aseptic technique and better ways/equipment for sampling. 5 minutes to look at plates Lecture continued Worksheet.

UNIT 1 Tissues, Organs, and Systems of Living Things.

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Cell Nutrients Nutrients required by cells can be classified in two categories: - are needed in concentrations larger than M. C, N, O, H, S, P, Mg.

Lec# 11 Plant tissue culture, GE Plants and applications

Plant Tissue Culture A rice plant growing in nutrient rich agar

Plant Tissue Culture.

Plant Tissue Cultures.

Lecture 8 Plant Tissue Culture & Applications. What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory”

Biotechnology – Biotechnological techniques

Introduction to Lab 6: Ex. Preparation of Culture Media

Structure, function and growth of prokaryote and eukaryote cells (iii) Differentiation of cells into tissues and organs. (iv) Cell and tissue culture –

Nutrition Life Science Grade 7.

Tissue Culture Unit one Cell and Molecular Biology Advanced Higher Biology.

Unit 1 Cell and Molecular Biology Section 3 Cell, tissue and plant tissue culture.

Structure, function and growth of prokaryote and eukaryote cells

Unit Plant Science. Problem Area Reproduction in Plants.

Horticulture Science Lesson 19 Propagating Plants by Tissue Culture

Plant Tissue Culture.

Nutrients Regulate body functions Promote growth Repair body tissue Obtain energy.

Working with yeast Microbiologists have developed techniques for isolating and maintaining strains in the laboratory Strains: microorganisms of the same.

Animal Nutrition. Need for Nourishment body processes require the use of energy obtained from ingested food or stored fat animal must have food to store.

PRINCIPLES OF CROP PRODUCTION ABT-320 (3 CREDIT HOURS))

Plant Tissue Culture Project. What is Plant Tissue Culture? Plant tissue culture is a form of asexual propagation of plants under laboratory conditions.

Basic Principles & Protocol in Plant Tissue Culture

Propagating Plants by Tissue Culture

Microbiology / Lab. 8. o Culture (Growth) Media I.What is a medium (plural media)? II.What is culture medium? III.What is meant by Inoculation of Media?

CONTENT INTRODUCTION REQUIREMENT PROCEDURE IMPORTANCE DISADVANTAGES

The Three Pillars of Yield

Tissue Engineering. Plant cells: current interest pharmaceuticals: atropine, codeine, L-dopa, morphine, phytotoxin (digitalis), taxol, ubiquinone-10,

DIFFERENTIATE BETWEEN PLANT, ANIMAL AND MICROBIAL BIOREACTOR

What is Tissue Culture A very technical method of asexual propagation The growing of plantlets from small pieces of plant tissue from a parent plant.

National 5 Biology Course Notes Unit 1 : Cell Biology Part 3 : Producing new cells.

Cell Culture Medium. Since 1950s, tissue culture media were developed and conditions were worked out which closely simulate the situation in vivo. Since.

Tissue Culture Tissue Culture is a method in which a few cells from the vegetative plant body are taken and kept in a nutrient medium.

Synthetic and natural media:

Conditions for Cell Culture

Title: Clones in nature

Plant Tissue Culture Media: Types, Constituents, Preparation and Selection Read this article to learn about the plant tissue culture media and its types,

Miss Minix Tarleton State University

AIM: To prepare the Nutrient Agar (solid) and Nutrient Broth (Liquid) medium.

To supply the growth requirements

Matter Tracing Process Tools for Lessons 10 and 11

Fruit trees Plant tissue culture General information

Culture Media for growing cells

Tools of the Laboratory: Methods for Culturing of Microorganisms

Molecular Techniques and Biotechnology

MATERIALS: Murashige & Skoog Medium (MS) Measuring cylinders

Internal Factors Affecting Plant Growth

Plant Tissue Culture & Animal Tissue Culture

Plant Tissue Culture Do you want a footer?.

What is a Culture Media? A growth media or a culture media is a liquid or gel designed to support the growth of micro organisms or cell.

Matter Tracing Process Tools for Lessons 10 and 11

& presents Plant Tissue Culture.

Metabolism and Survival

Micro-propagation Tissue Culture.

Plant Tissue Culture Do you want a footer?.

PLANTS (PHG 407) DR. DALIA RASHEED LECTURE (4)

Plant Tissue Culture?.

Presentation transcript:

Plant Tissue Culture

What is tissue culture????

What is tissue culture???? 1-Refers to technique of growing plant cells, tissues, organs, seeds 2-or other plant parts in a sterile environment on a nutrient medium

3-non-gene biotechnology to regenerate complete plantlet from isolated cells, or tissues or organ in an artificially prepared nutrient medium in the laboratory in aseptic condition.

Basic need of tissue culture 1-nutrient medium 2-aseptic condition 3-aeration

nutrient medium 1-"culture medium" or "Basal medium" or "growth medium" 2-depends on their nutrient medium composition 3-supplies energy and essential metabolites to growing tissues within it 4-state of culture medium solid = "Callus culture" or liquid = "Suspension culture"

Ingredients of nutrient medium 1-carbon source (e.g. sucrose, glucose, starch which supplies energy) 2-inorganic salts (major and minor elements), 3-vitamins, 4-hormones (auxin, cytokinin sometime GA).